• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 20514-1-AP

    WB analysis using 20514-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20514-1-AP (BTG4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 20514-1-AP

    IHC staining of human ovary using 20514-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 20514-1-AP (BTG4 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with BTG4 antibody included
  • IHC staining of human ovary using 20514-1-AP

    IHC staining of human ovary using 20514-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 20514-1-AP (BTG4 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with BTG4 antibody included

BTG4 Polyclonal antibody

BTG4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20514-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

B cell translocation gene 4, BTG family member 4, BTG4, PC3B, Protein BTG4, Protein PC3b

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inCaco-2 cells, HeLa cells, mouse colon tissue
Positive IHC detected inhuman ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy® BTG4 Ready-To-Use IHC Kit. BTG4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20514-1-AP targets BTG4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14339

Product name: Recombinant human BTG4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-171 aa of BC031045

Sequence: MRDEIATTVFFVTRLVKKHDKLSKQQIEDFAEKLMTILFETYRSHWHSDCPSKGQAFRCIRINNNQNKDPILERACVESNVDFSHLGLPKEMTIWVDPFEVCCRYGEKNHPFTVASFKGRWEEWELYQQISYAVSRASSDVSSGTSCDEESCSKEPRVIPKVSNPKSIYQV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 B-cell translocation gene 4
别名 B cell translocation gene 4, BTG family member 4, BTG4, PC3B, Protein BTG4, Protein PC3b
计算分子量 223 aa, 26 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC031045
基因名称 BTG4
Gene ID (NCBI) 54766
RRIDAB_10694822
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NY30
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BTG4 antibody 20514-1-APDownload protocol
IHC protocol for BTG4 antibody 20514-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols