验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, LNCaP cells, U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20336-1-AP targets BTG3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag14112 Product name: Recombinant human BTG3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 97-296 aa of BC011957 Sequence: DPCEVCCRRDGVSPCWPDCSQTPDLVIRPPWPPKALDYRREPLRPASSFLIMYGEKNNAFIVASFENKDENKDEISRKVTRALDKVTSDYHSGSSSSDEETSKEMEVKPSSVTAAASPVYQISELIFPPLPMWHPLPRKKPGMYRGNGHQNHYPPPVPFGYPNQGRKNKPYRPIPVTWVPPPGMHCDRNHWINPHMLAPH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | BTG family, member 3 |
| 别名 | TOB5, Protein Tob5, Protein BTG3, BTG family, member 3, BTG family member 3 |
| 计算分子量 | 296 aa, 34 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC011957 |
| 基因名称 | BTG3 |
| Gene ID (NCBI) | 10950 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14201 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BTG3 (B-cell translocation gene 3) is a member of the antiproliferative BTG/Tob gene family, which also includes BTG1, BTG2, BTG4, TOB, and TOB2. This gene family is characterized by similar N-terminal domains but divergent C-termini. BTG3 is considered a candidate tumor suppressor gene and plays a crucial role in various cellular processes, including cell proliferation, differentiation, and apoptosis. (PMID: 38714880)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BTG3 antibody 20336-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
