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验证数据展示

  • WB analysis using 16500-1-AP

    WB analysis using 16500-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16500-1-AP (BTF3L4 antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • IHC staining of rat brain using 16500-1-AP

    IHC staining of rat brain using 16500-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16500-1-AP (BTF3L4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BTF3L4 antibody included
  • IHC staining of rat brain using 16500-1-AP

    IHC staining of rat brain using 16500-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16500-1-AP (BTF3L4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BTF3L4 antibody included

BTF3L4 Polyclonal antibody

BTF3L4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16500-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

BTF3L4, RP4 800M22.5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, Jurkat cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy BTF3L4 Ready-To-Use IHC Kit. BTF3L4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

16500-1-AP targets BTF3L4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 BTF3L4 fusion protein Ag9666 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 basic transcription factor 3-like 4
别名 BTF3L4, RP4 800M22.5
计算分子量 158 aa, 17 kDa
观测分子量17-20 kDa
GenBank蛋白编号BC022371
基因名称 BTF3L4
Gene ID (NCBI) 91408
RRIDAB_2878265
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96K17
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BTF3L4 antibody 16500-1-APDownload protocol
IHC protocol for BTF3L4 antibody 16500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

Genome-wide association studies identify miRNA-194 as a prognostic biomarker for gastrointestinal cancer by targeting ATP6V1F, PPP1R14B, BTF3L4 and SLC7A5

Authors - Pan Huang
View Article
humanWB

Biol Direct

MIR194-2HG, a miRNA host gene activated by HNF4A, inhibits gastric cancer by regulating microRNA biogenesis

Authors - Hong Cao
View Article