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BTF3L4 Polyclonal antibody

BTF3L4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16500-1-AP

Print datasheet

Synonyms

BTF3L4, RP4 800M22.5

验证数据展示

  • WB analysis using 16500-1-AP

    WB analysis using 16500-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16500-1-AP (BTF3L4 antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • IHC staining of rat brain using 16500-1-AP

    IHC staining of rat brain using 16500-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16500-1-AP (BTF3L4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 16500-1-AP

    IHC staining of rat brain using 16500-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16500-1-AP (BTF3L4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293T cells, Jurkat cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16500-1-AP targets BTF3L4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen BTF3L4 fusion protein Ag9666 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name basic transcription factor 3-like 4
Synonyms BTF3L4, RP4 800M22.5
Calculated Molecular Weight 158 aa, 17 kDa
Observed Molecular Weight17-20 kDa
GenBank Accession NumberBC022371
Gene Symbol BTF3L4
Gene ID (NCBI) 91408
RRIDAB_2878265
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96K17
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BTF3L4 antibody 16500-1-APDownload protocol
IHC protocol for BTF3L4 antibody 16500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

Genome-wide association studies identify miRNA-194 as a prognostic biomarker for gastrointestinal cancer by targeting ATP6V1F, PPP1R14B, BTF3L4 and SLC7A5

Authors - Pan Huang
View Article