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验证数据展示

  • WB analysis using 16330-1-AP (same clone as 16330-1-PBS)

    WB analysis using 16330-1-AP (same clone as 16330-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16330-1-AP (BTD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16330-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 16330-1-AP (same clone as 16330-1-PBS)

    IHC staining of human liver using 16330-1-AP (same clone as 16330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16330-1-AP (BTD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16330-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 16330-1-AP (same clone as 16330-1-PBS)

    IHC staining of human liver using 16330-1-AP (same clone as 16330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16330-1-AP (BTD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16330-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 16330-1-AP (same clone as 16330-1-PBS)

    IHC staining of mouse kidney using 16330-1-AP (same clone as 16330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16330-1-AP (BTD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16330-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 16330-1-AP (same clone as 16330-1-PBS)

    IF Staining of HeLa using 16330-1-AP (same clone as 16330-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BTD antibody (16330-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16330-1-PBS in a different storage buffer formulation.

BTD Polyclonal antibody, PBS Only

BTD Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16330-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EC:3.5.1.12, biotinidase, Biotinase

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16330-1-PBS targets BTD in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9449

Product name: Recombinant human BTD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 194-543 aa of BC012099

Sequence: FNTNVVFSNNGTLVDRYRKHNLYFEAAFDVPLKVDLITFDTPFAGRFGIFTCFDILFFDPAIRVLRDYKVKHVVYPTAWMNQLPLLAAIEIQKAFAVAFGINVLAANVHHPVLGMTGSGIHTPLESFWYHDMENPKSHLIIAQVAKNPVGLIGAENATGETDPSHSKFLKILSGDPYCEKDAQEVHCDEATKWNVNAPPTFHSEMMYDNFTLVPVWGKEGYLHVCSNGLCCYLLYERPTLSKELYALGVFDGLHTVHGTYYIQVCALVRCGGLGFDTCGQEITEATGIFEFHLWGNFSTSYIFPLFLTSGMTLEVPDQLGWENDHYFLRKSRLSSGLVTAALYGRLYERD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 biotinidase
别名 EC:3.5.1.12, biotinidase, Biotinase
计算分子量 543 aa, 61 kDa
观测分子量61 kDa
GenBank蛋白编号BC012099
基因名称 BTD
Gene ID (NCBI) 686
RRIDAB_2067512
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43251
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BTD(Biotinidase) functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. BTD has also been shown to have biotinyl transferase activity.

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