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验证数据展示

  • WB analysis of HepG2 using 13776-1-AP

    WB analysis of HepG2 using 13776-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13776-1-AP (BTBD6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of Y79 using 13776-1-AP

    IP experiment of Y79 using 13776-1-AP

    IP result of anti-BTBD6 (IP:13776-1-AP, 4ug; Detection:13776-1-AP 1:300) with Y79 cells lysate 800ug.

BTBD6 Polyclonal antibody

BTBD6 Polyclonal Antibody for WB, IP, ELISA
Cat No. 13776-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

BDPL, BTB (POZ) domain containing 6, BTBD6, Lens BTB domain protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inY79 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13776-1-AP targets BTBD6 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BTBD6 fusion protein Ag4766 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BTB (POZ) domain containing 6
别名 BDPL, BTB (POZ) domain containing 6, BTBD6, Lens BTB domain protein
计算分子量 410 aa, 46 kDa
观测分子量 53-58 kDa
GenBank蛋白编号BC042525
基因名称 BTBD6
Gene ID (NCBI) 90135
RRIDAB_2877975
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96KE9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BTBD6 antibody 13776-1-APDownload protocol
IP protocol for BTBD6 antibody 13776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols