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验证数据展示

  • WB analysis of HepG2 using 13776-1-AP

    WB analysis of HepG2 using 13776-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13776-1-AP (BTBD6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of Y79 using 13776-1-AP

    IP experiment of Y79 using 13776-1-AP

    IP result of anti-BTBD6 (IP:13776-1-AP, 4ug; Detection:13776-1-AP 1:300) with Y79 cells lysate 800ug.

BTBD6 Polyclonal antibody

BTBD6 Polyclonal Antibody for WB, IP, ELISA
Cat No. 13776-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

BDPL, BTB (POZ) domain containing 6, BTBD6, Lens BTB domain protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inY79 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13776-1-AP targets BTBD6 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4766

Product name: Recombinant human BTBD6 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-410 aa of BC042525

Sequence: MFNNELMADVHFVVGPPGATRTVPAHKYVLAVGSSVFYAMFYGDLAEVKSEIHIPDVEPAAFLILLKYMYSDEIDLEADTVLATLYAAKKYIVPALAKACVNFLETSLEAKNACVLLSQSRLFEEPELTQRCWEVIDAQAEMALRSEGFCEIDRQTLEIIVTREALNTKEAVVFEAVLNWAEAECKRQGLPITPRNKGHVLGRALYLVRIPTMTLEEFANGAAQSDILTLEETHSIFLWYTATNKPRLDFPLTKRKGLAPQRCHRFQSSAYRSNQWRYRGRCDSIQFAVDRRVFIAGLGLYGSSSGKAEYSVKIELKRLGVVLAQNLTKFMSDGSSNTFPVWFEHPVQVEQDTFYTASAVLDGSELSYFGQEGMTEVQCGKVAFQFQCSSDSTNGTGVQGGQIPELIFYA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BTB (POZ) domain containing 6
别名 BDPL, BTB (POZ) domain containing 6, BTBD6, Lens BTB domain protein
计算分子量 410 aa, 46 kDa
观测分子量 53-58 kDa
GenBank蛋白编号BC042525
基因名称 BTBD6
Gene ID (NCBI) 90135
RRIDAB_2877975
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96KE9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IP protocol for BTBD6 antibody 13776-1-APDownload protocol
WB protocol for BTBD6 antibody 13776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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