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验证数据展示

  • IHC staining of human kidney using 24733-1-AP

    IHC staining of human kidney using 24733-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24733-1-AP (BTBD16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse testis using 24733-1-AP

    WB analysis of mouse testis using 24733-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24733-1-AP (BTBD16 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

BTBD16 Polyclonal antibody

BTBD16 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24733-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

BTB (POZ) domain containing 16, BTBD16, C10orf87

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24733-1-AP targets BTBD16 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 BTBD16 fusion protein Ag20672 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BTB (POZ) domain containing 16
别名 BTB (POZ) domain containing 16, BTBD16, C10orf87
计算分子量 507 aa, 59 kDa
观测分子量 60 kDa
GenBank蛋白编号BC109262
基因名称 BTBD16
Gene ID (NCBI) 118663
RRIDAB_2879696
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ32M84
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BTBD16 also named as BTB (POZ) domain containing 16 or C10orf87 is 506 amino acid protein, which one BTB domain. The molecular weight fo BTBD16 is 58kDa. The function of BTBD16 protein is still non-known.

实验方案

Product Specific Protocols
WB protocol for BTBD16 antibody 24733-1-APDownload protocol
IHC protocol for BTBD16 antibody 24733-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols