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验证数据展示

  • WB analysis using 13557-1-AP

    WB analysis using 13557-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13557-1-AP (BRUNOL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 13557-1-AP

    IHC staining of mouse brain using 13557-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13557-1-AP (BRUNOL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 13557-1-AP

    IHC staining of rat brain using 13557-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13557-1-AP (BRUNOL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-87 MG using 13557-1-AP

    IF Staining of U-87 MG using 13557-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using BRUNOL5 antibody (13557-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

BRUNOL5 Polyclonal antibody

BRUNOL5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13557-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CELF-5, CELF5, CELF 5, Bruno-like protein 5, BRUNOL 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, U-251 cells
Positive IHC detected inmouse brain tissue, human gliomas tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-87 MG cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13557-1-AP targets BRUNOL5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4416

Product name: Recombinant human BRUNOL5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 135-485 aa of BC028101

Sequence: KLFVGMLNKQQSEEDVLRLFQPFGVIDECTVLRGPDGSSKGCAFVKFSSHTEAQAAIHALHGSQTMPGASSSLVVKFADTDKERTLRRMQQMVGQLGILTPSLTLPFSPYSAYAQALMQQQTTVLSTSGSYLSPGVAFSPCHIQQIGAVSLNGLPATPIAPASGLHSPPLLGTTAVPGLVAPITNGFAGVVPFPGGHPALETVYANGLVPYPAQSPTVAETLHPAFSGVQQYTAMYPTAAITPIAHSVPQPPPLLQQQQREGPEGCNLFIYHLPQEFGDTELTQMFLPFGNIISSKVFMDRATNQSKCFGFVSFDNPASAQAAIQAMNGFQIGMKRLKVQLKRPKDPGHPY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bruno-like 5, RNA binding protein (Drosophila)
别名 CELF-5, CELF5, CELF 5, Bruno-like protein 5, BRUNOL 5
计算分子量 485 aa, 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC028101
基因名称 BRUNOL5
Gene ID (NCBI) 60680
RRIDAB_1211624
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N6W0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRUNOL5, also named CELF5, is a 485 amino acid protein, which belongs to the CELF/BRUNOL family. BRUNOL5 is expressed in the brain and localizes in the nucleus. BRUNOL5 is an RNA-binding protein implicated in the regulation of pre-mRNA alternative splicing and mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. BRUNOL5 specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. BRUNOL5 exists in two isoforms with MV 52 and 45 kDa.

实验方案

Product Specific Protocols
WB protocol for BRUNOL5 antibody 13557-1-APDownload protocol
IHC protocol for BRUNOL5 antibody 13557-1-APDownload protocol
IF protocol for BRUNOL5 antibody 13557-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols