验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse testis tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11589-1-AP targets BRSK2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IP, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | BRSK2 fusion protein Ag2195 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | BR serine/threonine kinase 2 |
| 别名 | BR serine/threonine kinase 2, BRSK2, C11orf7, PEN11B, SAD1, STK29 |
| 计算分子量 | 87 kDa |
| 观测分子量 | 80-88 kDa |
| GenBank蛋白编号 | BC024291 |
| 基因名称 | BRSK2 |
| Gene ID (NCBI) | 9024 |
| RRID | AB_2243660 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IWQ3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BRSK2 is highly conserved from invertebrates to human, plays a key role in polarization of neurons and has been identified with different names: SAD1 is its closest homolog in C. elegans, serine/threonine kinase SADA alfa in rat, brain-selective kinase 2 and SAD-B in mouse and hsSAD1 and SAD1B in humans(PMID:16165222) . In rat and mouse, BRSK2 is only detected in the brain and at low levels in the testis .It has some isoforms produced by alternative splicing with the molecular mass of 68-84 kDa. This protein has a conserved function throughout the evolution.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BRSK2 antibody 11589-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem Specification of neuronal polarity regulated by local translation of CRMP2 and Tau via the mTOR-p70S6K pathway. | ||
J Neurochem Phosphorylation of microtubule-associated protein tau by AMPK-related kinases. | ||
Endocrinology SAD-A Promotes Glucose-Stimulated Insulin Secretion Through Phosphorylation and Inhibition of GDIα in Male Islet β Cells.
| ||
Cancer Lett SOD1-high fibroblasts derived exosomal miR-3960 promotes cisplatin resistance in triple-negative breast cancer by suppressing BRSK2-mediated phosphorylation of PIMREG
|
