• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

BRD9 Polyclonal antibody

BRD9 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 24785-1-AP

Print datasheet

Synonyms

BRD9, bromodomain containing 9, LAVS3040, PRO9856

验证数据展示

  • WB analysis of HeLa using 24785-1-AP

    WB analysis of HeLa using 24785-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24785-1-AP (BRD9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 24785-1-AP

    IHC staining of human colon cancer using 24785-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24785-1-AP (BRD9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 24785-1-AP

    IHC staining of human colon cancer using 24785-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24785-1-AP (BRD9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24785-1-AP targets BRD9 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IP, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen BRD9 fusion protein Ag20427 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bromodomain containing 9
Synonyms BRD9, bromodomain containing 9, LAVS3040, PRO9856
Calculated Molecular Weight 597 aa, 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC041590
Gene Symbol BRD9
Gene ID (NCBI) 65980
RRIDAB_2879722
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H8M2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRD9, also named as MU-RMS-40.8, may play a role in chromatin remodeling and regulation of transcription. BRD9 has six isoforms with MW 67 kDa, 23 kDa, 53 kDa, 26 kDa, 56 kDa and 61 kDa.

实验方案

Product Specific Protocols
WB protocol for BRD9 antibody 24785-1-APDownload protocol
IHC protocol for BRD9 antibody 24785-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Discov

A role for SMARCB1 in synovial sarcomagenesis reveals that SS18-SSX induces canonical BAF destruction.

Authors - Jinxiu Li
View Article
humanWB

Nat Commun

SMARCB1 loss activates patient-specific distal oncogenic enhancers in malignant rhabdoid tumors

Authors - Ning Qing Liu
View Article
humanIP,IF

PLoS Pathog

Merkel cell polyomavirus small T antigen is a viral transcription activator that is essential for viral genome maintenance

Authors - Kyle Rapchak
View Article