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验证数据展示

  • WB analysis of HeLa using 60121-1-Ig (same clone as 60121-1-PBS)

    WB analysis of HeLa using 60121-1-Ig (same clone as 60121-1-PBS)

    WB result of BRD8 antibody (60121-1-Ig, 1:8000) with si-Control and si-BRD8 transfected HeLa cells.. This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

  • WB analysis of fetal human brain using 60121-1-Ig (same clone as 60121-1-PBS)

    WB analysis of fetal human brain using 60121-1-Ig (same clone as 60121-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60121-1-Ig (BRD8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 60121-1-Ig (same clone as 60121-1-PBS)

    IHC staining of human colon cancer using 60121-1-Ig (same clone as 60121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60121-1-Ig (BRD8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60121-1-Ig (same clone as 60121-1-PBS)

    IF Staining of HepG2 using 60121-1-Ig (same clone as 60121-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BRD8 antibody (60121-1-Ig, Clone: 5B6D6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60121-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

BRD8 Monoclonal antibody, PBS Only

BRD8 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60121-1-PBS

产品说明书

CloneNo. 5B6D6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

BRD8, bromodomain containing 8, p120, SMAP, SMAP2, TrCP120

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

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产品信息

60121-1-PBS targets BRD8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BRD8 fusion protein Ag0790 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 bromodomain containing 8
别名 BRD8, bromodomain containing 8, p120, SMAP, SMAP2, TrCP120
计算分子量 120 kDa
观测分子量 120 kDa
GenBank蛋白编号BC008039
基因名称 BRD8
Gene ID (NCBI) 10902
RRIDAB_2066025
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H0E9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine- binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. It is also reported to be an accessory subunit of NuA4 HAT complex (also known as TRRAP/TIP60 complex) through biochemical purification [PMID: 16049979]. In addition, it can bind to thyroid hormone receptor-ß and/or retinoid X receptor, and can act as a coactivator of the nuclear hormone receptor-mediated transcription in reporter assays [PMID: 10517671].