验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse lung tissue, PC-3 cells, SH-SY5Y cells, COLO 320 cells |
Planning an IHC experiment? We recommend our IHCeasy BRD7 Ready-To-Use IHC Kit. BRD7 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86176-2-RR targets BRD7 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | BRD7 fusion protein Ag1461 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | bromodomain containing 7 |
| 别名 | BP75, Bromodomain-containing protein 7, CELTIX1, NAG4, Protein CELTIX 1 |
| 计算分子量 | 651 aa, 74 kDa |
| 观测分子量 | 74-78 kDa |
| GenBank蛋白编号 | BC050728 |
| 基因名称 | BRD7 |
| Gene ID (NCBI) | 29117 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9NPI1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BRD7 (bromodomain containing 7), is a subunit of poly-bromo-associated BRG1-associated factor (PBAF)-specific Swi/Snf chromatin remodeling complexes.BRD7 is proposed as a tumor suppressor protein, since its identification as an important component of both functional p53 and BRCA1 (breast cancer 1, early onset) pathways. BRD7 could down-regulate the GSK3beta phosphotransferase activity as dephosphorylation of GSK3B at 'Tyr-216', thus activate the Wnt signaling pathway in a DVL1-dependent manner.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BRD7 antibody 86176-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |