验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, Jurkat cells, MCF-7 cells, MDA-MB-231 cells, NIH/3T3 cells, C6 cells |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy BRD4 Ready-To-Use IHC Kit. BRD4 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
88289-2-RR targets BRD4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | bromodomain containing 4 |
| 别名 | BRD 4, bromodomain containing 4, Bromodomain-containing protein 4, HUNKI, MCAP |
| 计算分子量 | 1362 aa, 152 kDa |
| 观测分子量 | 200 kDa |
| GenBank蛋白编号 | BC035266 |
| 基因名称 | BRD4 |
| Gene ID (NCBI) | 23476 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O60885 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BRD4 is a member of the BET (bromodomain and extra terminal domain) family, which also includes BRD2, BRD3, and BRDT. BRD4, similar to other BET family members, contains two bromodomains that recognize acetylated lysine residues. BRD4 also has an extended C-terminal domain with little sequence homology to other BET family members. BRD4 has some isoforms, the long isoform BRD4-L has an extended C terminus, while the short isoform BRD4-S lacks this C-terminal extension. The molecular weight range of BRD4-S and BRD4-L is 110 kDa to 200 kDa (PMID: 32203489).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for BRD4 antibody 88289-2-RR | Download protocol |
| WB protocol for BRD4 antibody 88289-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
