验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, NIH/3T3 cells, HepG2 cells, LNcaP cells, Jurkat cells, K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy BRD4 Ready-To-Use IHC Kit. BRD4 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| ChIP | See 2 publications below |
产品信息
67374-2-Ig targets BRD4 in WB, ChIP, ELISA applications and shows reactivity with Human, Mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, ChIP |
| 经测试反应性 | Human, Mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag27545 Product name: Recombinant human BRD4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 311-633 aa of BC035266 Sequence: PEPKTTKLGQRRESSRPVKPPKKDVPDSQQHPAPEKSSKVSEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVAVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKRKEEVEENKKSKAKEPPPKKTKKNNSSNSNVSKKEPAPMKSKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRV 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | bromodomain containing 4 |
| 别名 | BRD4, bromodomain containing 4, CAP, HUNK1, HUNKI, MCAP, Protein HUNK1 |
| 计算分子量 | 1362 aa, 152 kDa |
| 观测分子量 | 200 kDa |
| GenBank蛋白编号 | BC035266 |
| 基因名称 | BRD4 |
| Gene ID (NCBI) | 23476 |
| RRID | AB_2918488 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O60885 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BRD4 antibody 67374-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Bioengineered Pathological stage-associated non-coding RNA long intergenic non-protein coding RNA 1234 (LINC01234) participation in cell cycle regulation in adrenocortical carcinoma through bromodomain-containing protein 4 (BRD4) expression mediation via sponging microRNA (miR)-140-3p. | ||
Curr Med Sci ARID1A Inactivation Increases Expression of circ0008399 and Promotes Cisplatin Resistance in Bladder Cancer | ||
J Transl Med Vascular restenosis following paclitaxel-coated balloon therapy is attributable to NLRP3 activation and LIN9 upregulation | ||
Nat Commun MMP-2-triggered, mitochondria-targeted PROTAC-PDT therapy of breast cancer and brain metastases inhibition | ||
Tissue Cell Bromodomain containing 4 inhibition combats gastric precancerous lesions via modulating macrophage polarization
| ||
Cell De novo assembly of nuclear stress bodies rearranges and enhances NFIL3 to restrain acute inflammatory responses |