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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85990-2-RR (same clone as 85990-2-PBS)

    WB analysis using 85990-2-RR (same clone as 85990-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85990-2-RR (BRD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85990-2-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 85990-2-RR (same clone as 85990-2-PBS)

    IP experiment of HeLa using 85990-2-RR (same clone as 85990-2-PBS)

    IP result of anti-BRD2 (IP:85990-2-RR, 4ug; Detection:85990-2-RR 1:3000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 85990-2-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 85990-2-RR (same clone as 85990-2-PBS)

    IF Staining of Jurkat using 85990-2-RR (same clone as 85990-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using BRD2 antibody (85990-2-RR, Clone: 250453G5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85990-2-PBS in a different storage buffer formulation.

BRD2 Recombinant antibody, PBS Only

BRD2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 85990-2-PBS

产品说明书

CloneNo. 250453G5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

bromodomain containing 2, Bromodomain-containing protein 2, D6S113E, FSH, FSRG1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85990-2-PBS targets BRD2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BRD2 fusion protein Ag17589 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 bromodomain containing 2
别名 bromodomain containing 2, Bromodomain-containing protein 2, D6S113E, FSH, FSRG1
计算分子量 836 aa, 92 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC063840
基因名称 BRD2
Gene ID (NCBI) 6046
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25440
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BRD2 (Bromodomain-containing protein 2), also known as RING3, is a member of the Bromodomain and Extra-Terminal (BET) family of proteins. These proteins contain two bromodomains and one extra-terminal domain. BRD2 primarily localizes to the nucleus and functions as an atypical protein kinase. Its main role involves the activation of E2F1 and E2F2 proteins, facilitating the production of proteins essential for the G1/S phase transition of the cell cycle. BRD2 specifically recognizes acetylated histones through its bromodomains, particularly acetylated lysine residues on histones H3 and H4. This interaction allows BRD2 to recruit various transcription factors, co-activators, and repressors, thereby modulating transcriptional activity. BRD2 plays a crucial role in chromatin remodeling by recruiting proteins such as histone deacetylases. It is highly expressed during neural tube development and closure, making it essential for neurogenesis and embryogenesis.(PMID: 33462181, PMID: 37926722)