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验证数据展示

  • WB analysis of HEK-293 using 16461-1-AP

    WB analysis of HEK-293 using 16461-1-AP

    HEK-293 and Hela cells were subjected to SDS PAGE followed by western blot with 16461-1-AP (BPNT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16461-1-AP

    IHC staining of human liver using 16461-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16461-1-AP (BPNT1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 16461-1-AP

    IHC staining of human liver using 16461-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16461-1-AP (BPNT1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 16461-1-AP

    IF Staining of HeLa using 16461-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16461-1-AP (BPNT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

BPNT1 Polyclonal antibody

BPNT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16461-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Bisphosphate 3 nucleotidase 1, BPNT1, PAP inositol 1,4 phosphatase, PIP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, human kidney tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16461-1-AP targets BPNT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性rat
免疫原 BPNT1 fusion protein Ag9523 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 3'(2'), 5'-bisphosphate nucleotidase 1
别名 Bisphosphate 3 nucleotidase 1, BPNT1, PAP inositol 1,4 phosphatase, PIP
计算分子量 325 aa, 36 kDa
观测分子量 36-40 kDa
GenBank蛋白编号BC017801
基因名称 BPNT1
Gene ID (NCBI) 10380
RRIDAB_2067193
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95861
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BPNT1 antibody 16461-1-APDownload protocol
IHC protocol for BPNT1 antibody 16461-1-APDownload protocol
IF protocol for BPNT1 antibody 16461-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

PLoS One

Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea

Authors - Jing Liu
View Article