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验证数据展示

  • WB analysis of A549 using 13461-2-AP

    WB analysis of A549 using 13461-2-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 13461-2-AP

    IF Staining of A549 using 13461-2-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using BPIL1 antibody (13461-2-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • WB analysis using 13461-2-AP

    WB analysis using 13461-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13461-2-AP (BPIL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 13461-2-AP

    IHC staining of human tonsillitis using 13461-2-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13461-2-AP (BPIL1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BPIL1 Polyclonal antibody

BPIL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13461-2-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Bactericidal/permeability-increasing protein-like 1, BPI fold-containing family B member 2, BPIFB2, BPI-like 1, C20orf184

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13461-2-AP targets BPIL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 BPIL1 fusion protein Ag4051 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bactericidal/permeability-increasing protein-like 1
别名 Bactericidal/permeability-increasing protein-like 1, BPI fold-containing family B member 2, BPIFB2, BPI-like 1, C20orf184
计算分子量 458 aa, 49 kDa
观测分子量 49 kDa
GenBank蛋白编号BC034415
基因名称 BPIL1
Gene ID (NCBI) 80341
RRIDAB_2067089
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N4F0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Bactericidal/permeability-increasing protein-like 1 (BPIL1, also known as LPLUNC2 or BPIFB2) is a member of the lipid transfer (LT)/lipopolysaccharide binding protein (LBP) family. LT/LBP proteins are structurally related proteins capable of binding phospholipids and lipopolysaccharides (PMID: 12185532). The gene of BPIL1 maps to Chromosome 20q11, and encodes a 458-amino acid protein with a calculated molecular weight of 49 kDa. It is highly expressed in hypertrophic tonsils. BPIL1 has been identified as a nasal mucous protein that may be involved in immune response in the nose against microbial infections (PMID: 15996010). Reduced expression of antimicrobial PLUNC proteins (LPLUNC1 and LPLUNC2) has been reported in nasal polyp tissues of patients with chronic rhinosinusitis (PMID: 22676062).

实验方案

Product Specific Protocols
WB protocol for BPIL1 antibody 13461-2-APDownload protocol
IHC protocol for BPIL1 antibody 13461-2-APDownload protocol
IF protocol for BPIL1 antibody 13461-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Allergy

Reduced expression of antimicrobial PLUNC proteins in nasal polyp tissues of patients with chronic rhinosinusitis.

Authors - Seshadri S S
View Article
humanWB

J Proteome Res

Proteomic analysis of human bronchoalveolar lavage fluid after subsgemental exposure.

Authors - Foster Matthew W MW
View Article
humanIHC

J Immunol Res

Reduced Expression of Antimicrobial Protein Secretory Leukoprotease Inhibitor and Clusterin in Chronic Rhinosinusitis with Nasal Polyps.

Authors - Yanran Huang
View Article