验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, human plasma, mouse liver tissue, rat liver tissue, HeLa cells, Jurkat cells, K-562 cells, LNCaP cells, U-87 MG cells, mouse heart tissue, mouse lung tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17173-1-AP targets BPGM in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag10731 Product name: Recombinant human BPGM protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-259 aa of BC017050 Sequence: MSKYKLIMLRHGEGAWNKENRFCSWVDQKLNSEGMEEARNCGKQLKALNFEFDLVFTSVLNRSIHTAWLILEELGQEWVPVESSWRLNERHYGALIGLNREQMALNHGEEQVRLWRRSYNVTPPPIEESHPYYQEIYNDRRYKVCDVPLDQLPRSESLKDVLERLLPYWNERIAPEVLRGKTILISAHGNSSRALLKHLEGISDEDIINITLPTGVPILLELDENLRAVGPHQFLGDQEAIQAAIKKVEDQGKVKQAKK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 2,3-bisphosphoglycerate mutase |
| 别名 | 2,3 bisphosphoglycerate mutase, Bisphosphoglycerate mutase, BPG dependent PGAM, BPGM |
| 计算分子量 | 259 aa, 30 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | BC017050 |
| 基因名称 | BPGM |
| Gene ID (NCBI) | 669 |
| RRID | AB_2274991 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P07738 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BPGM antibody 17173-1-AP | Download protocol |
| IHC protocol for BPGM antibody 17173-1-AP | Download protocol |
| IP protocol for BPGM antibody 17173-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Erythrocyte transglutaminase-2 combats hypoxia and chronic kidney disease by promoting oxygen delivery and carnitine homeostasis. | ||
Brain Res Bull Enhanced BPGM/2,3-DPG pathway activity suppresses glycolysis in hypoxic astrocytes via FIH-1 and TET2 | ||
Front Physiol Alteration in the number, morphology, function, and metabolism of erythrocytes in high-altitude polycythemia | ||
