验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, human plasma, mouse liver tissue, rat liver tissue, HeLa cells, Jurkat cells, K-562 cells, LNCaP cells, U-87 MG cells, mouse heart tissue, mouse lung tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17173-1-AP targets BPGM in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag10731 Product name: Recombinant human BPGM protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-259 aa of BC017050 Sequence: MSKYKLIMLRHGEGAWNKENRFCSWVDQKLNSEGMEEARNCGKQLKALNFEFDLVFTSVLNRSIHTAWLILEELGQEWVPVESSWRLNERHYGALIGLNREQMALNHGEEQVRLWRRSYNVTPPPIEESHPYYQEIYNDRRYKVCDVPLDQLPRSESLKDVLERLLPYWNERIAPEVLRGKTILISAHGNSSRALLKHLEGISDEDIINITLPTGVPILLELDENLRAVGPHQFLGDQEAIQAAIKKVEDQGKVKQAKK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 2,3-bisphosphoglycerate mutase |
| 别名 | 2,3 bisphosphoglycerate mutase, 2,3-bisphosphoglycerate mutase, erythrocyte, 2,3-bisphosphoglycerate synthase, 2,3-diphosphoglycerate mutase, Bisphosphoglycerate mutase |
| 计算分子量 | 259 aa, 30 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | BC017050 |
| 基因名称 | BPGM |
| Gene ID (NCBI) | 669 |
| RRID | AB_2274991 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P07738 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BPGM is a cytosolic erythrocyte enzyme that controls intracellular levels of 2,3-bisphosphoglycerate, a key allosteric regulator of hemoglobin. By modulating hemoglobin oxygen affinity, BPGM ensures efficient oxygen delivery to tissues and supports physiological adaptation to hypoxia. Genetic disruption of BPGM leads to erythrocytosis and impaired oxygen release, underscoring its central role in red blood cell metabolic specialization. BPGM in HCC cells also induced both histone lactylation and M2 polarization of macrophages by lactate secretion. (PMID: 41514495)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for BPGM antibody 17173-1-AP | Download protocol |
| IP protocol for BPGM antibody 17173-1-AP | Download protocol |
| WB protocol for BPGM antibody 17173-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Erythrocyte transglutaminase-2 combats hypoxia and chronic kidney disease by promoting oxygen delivery and carnitine homeostasis. | ||
Brain Res Bull Enhanced BPGM/2,3-DPG pathway activity suppresses glycolysis in hypoxic astrocytes via FIH-1 and TET2 | ||
Front Physiol Alteration in the number, morphology, function, and metabolism of erythrocytes in high-altitude polycythemia | ||
Adv Sci (Weinh) Hepatocyte BPGM Induces RET Lactylation and Macrophage Reprogramming to Promote Tumorigenesis in Hepatocellular Carcinoma |
