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验证数据展示

  • WB analysis using 13720-1-AP (same clone as 13720-1-PBS)

    WB analysis using 13720-1-AP (same clone as 13720-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13720-1-AP (BOULE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

    IF Staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using BOULE antibody (13720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

    IP experiment of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

    IP result of anti-BOULE (IP:13720-1-AP, 4ug; Detection:13720-1-AP 1:1000) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

    IHC staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BOLL/BOULE antibody included
  • IF Staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

    IF Staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse testis tissue using 13720-1-AP (BOULE antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

BOULE Polyclonal antibody, PBS Only

BOULE Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA
Cat No. 13720-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

别名

BOLL, Protein boule-like

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BOLL/BOULE Ready-To-Use IHC Kit. BOLL/BOULE primary antibody included.

产品信息

13720-1-PBS targets BOULE in WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4662

Product name: Recombinant human BOLL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-283 aa of BC033674

Sequence: MQTDSLSPSPNPVSPVPLNNPTSAPRYGTVIPNRIFVGGIDFKTNESDLRKFFSQYGSVKEVKIVNDRAGVSKGYGFVTFETQEDAQKILQEAEKLNYKDKKLNIGPAIRKQQVGIPRSSIMPAAGTMYLTTSTGYPYTYHNGVAYFHTPEVTSVPPPWPSRSVCSSPVMVAQPIYQQPAYHYQATTQYLPGQWQWSVPQPSASSAPFLYLQPSEVIYQPVEIAQDGGCVPPPLSLMETSVPEPYSDHGVQATYHQVYAPSAITMPAPVMQPEPIKTVWSIHY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bol, boule-like (Drosophila)
别名 BOLL, Protein boule-like
计算分子量 283 aa, 31 kDa
观测分子量 38-42 kDa
GenBank蛋白编号BC033674
基因名称 BOULE
Gene ID (NCBI) 66037
RRIDAB_2290617
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N9W6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BOULE, also called BOLL, belongs to the RRM DAZ family. BOULE has been identified as being involved in regulating the development and differentiation of germ cells and playing an essential role in infertility (PMID: 31683986). BOULE is specifically expressed in testis, but not in early embryos, primordial germ cells, and spermatogonial cells. It is first expressed in the cytoplasm of spermatocytes and then persists through meiosis. BOULE has 5 isoforms with the molecular mass of 19 and 31-38 kDa. This antibody detects two bands in testis, the lower band of ~40 kDa in size corresponds to the predicted endogenous BOULE protein, while the upper band may be an isoform or posttranslationally modified version of the BOULE protein (PMID: 27632217).

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