验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293T cells, K-562 cells, HUVEC cells |
Planning an IHC experiment? We recommend our IHCeasy BORG5/CDC42EP1 Ready-To-Use IHC Kit. BORG5/CDC42EP1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27904-1-AP targets BORG5/CDC42EP1 in WB, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, canine |
| 免疫原 | BORG5/CDC42EP1 fusion protein Ag27396 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CDC42 effector protein (Rho GTPase binding) 1 |
| 别名 | BORG5, CDC42EP1, MSE55, CEP1, Cdc42 effector protein 1 |
| 计算分子量 | 40 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC009356 |
| 基因名称 | CDC42EP1 |
| Gene ID (NCBI) | 11135 |
| RRID | AB_3086008 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q00587 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDC42EP1, also known as BORG5, is a CDC42 binding protein that mediates actin cytoskeleton reorganization at the plasma membrane. This protein is secreted and is primarily found in bone marrow. BORG5 was discovered as a 55 kDa protein, but the accepted reference protein is only 40.3 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BORG5/CDC42EP1 antibody 27904-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |