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验证数据展示

  • WB analysis using 13262-1-AP

    WB analysis using 13262-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13262-1-AP (BNIPL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skin using 13262-1-AP

    IHC staining of mouse skin using 13262-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 13262-1-AP (BNIPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skin using 13262-1-AP

    IHC staining of mouse skin using 13262-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 13262-1-AP (BNIPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BNIPL Polyclonal antibody

BNIPL Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13262-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Bcl-2/adenovirus E1B 19 kDa-interacting protein 2-like protein, BNIP S, BNIPL 1, BNIPL 2, BNIPL1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skin tissue, rat skin tissue
Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13262-1-AP targets BNIPL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3921

Product name: Recombinant human BNIPL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-357 aa of BC027868

Sequence: MGTIQEAGKKTDVGVREIAEAPELGAALRHGELELKEEWQDEEFPRLLPEEAGTSEDPEDPKGDSQAAAGTPSTLALCGQRPMRKRLSAPELRLSLTKGPGNDGASPTQSAPSSPDGSSDLEIDELETPSDSEQLDSGHEFEWEDELPRAEGLGTSETAERLGRGCMWDVTGEDGHHWRVFRMGPREQRVDMTVIEPYKKVLSHGGYHGDGLNAVILFASCYLPRSSIPNYTYVMEHLFRYMVGTLELLVAENYLLVHLSGGTSRAQVPPLSWIRQCYRTLDRRLRKNLRALVVVHATWYVKAFLALLRPFISSKFTRKIRFLDSLGELAQLISLDQVHIPEAVRQLDRDLHGSGGT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BCL2/adenovirus E1B 19kD interacting protein like
别名 Bcl-2/adenovirus E1B 19 kDa-interacting protein 2-like protein, BNIP S, BNIPL 1, BNIPL 2, BNIPL1
计算分子量 357 aa, 40 kDa
观测分子量43 kDa
GenBank蛋白编号BC027868
基因名称 BNIPL
Gene ID (NCBI) 149428
RRIDAB_3085410
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z465
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BNIPL interacts with several other proteins, such as BCL2, ARHGAP1, MIF and GFER. It may function as a bridge molecule between BCL2 and ARHGAP1/CDC42 in promoting cell death.

实验方案

Product Specific Protocols
WB protocol for BNIPL antibody 13262-1-APDownload protocol
IHC protocol for BNIPL antibody 13262-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols