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验证数据展示

  • WB analysis using 68091-1-Ig (same clone as 68091-1-PBS)

    WB analysis using 68091-1-Ig (same clone as 68091-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 68091-1-Ig (same clone as 68091-1-PBS)

    IHC staining of mouse kidney using 68091-1-Ig (same clone as 68091-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 68091-1-Ig (same clone as 68091-1-PBS)

    IHC staining of mouse brain using 68091-1-Ig (same clone as 68091-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68091-1-Ig (same clone as 68091-1-PBS)

    IF Staining of HepG2 using 68091-1-Ig (same clone as 68091-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HepG2 cells using BNIP3 antibody (68091-1-Ig, Clone: 2H7D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

BNIP3 Monoclonal antibody, PBS Only

BNIP3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68091-1-PBS

产品说明书

CloneNo. 2H7D8

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

2H7D8, NIP3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68091-1-PBS targets BNIP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag27935

Product name: Recombinant human BNIP3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-194 aa of BC009342

Sequence: MQEESLQGSWVELHFSNNGNGGSVPASVSIYNGDMEKILLDAQHESGRSSSKSSHCDSPPRSQTPQDTNRASETDTHSIGEKNSSQSEEDDIERRKEVESILKKNSDWIWDWSSRPENIPPKEFLFKHPKRTATLSMRNTSVMKKGGIFSAEFLKVFLPSLLLSHLLAIGLGIYIGRRLTTSTSTF

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 BCL2/adenovirus E1B 19kDa interacting protein 3
别名 2H7D8, NIP3
计算分子量 194 aa, 22 kDa
观测分子量25-30,50-60 kDa
GenBank蛋白编号BC009342
基因名称 BNIP3
Gene ID (NCBI) 664
RRIDAB_2918828
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ12983
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BNIP3 (BCL2 interacting protein 3) is a member of the Bcl-2 family that is expressed in mitochondria and induces apoptosis (PMID: 10891486). It can regulate the permeabillity state of outer mitchondrial member (OMM), this regulation is accompanied by the formation of homo- and hetero-oligomers inside the membrane. Hypoxia can lead to transcriptional activation of BNIP3 through HIF-1α (PMID: 11559532). It's calculated molecule weight is 21.5 kDa. The antibody can detect bands around 25 and 50 kDa, representing monomeric and dimeric forms, respectively (PMID: 21364626).BNIP3 is related to the occurrence and development of tumors, cardiovascular diseases, and Parkinson's diseases.

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