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验证数据展示

  • WB analysis using 10361-1-AP

    WB analysis using 10361-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10361-1-AP (BNIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 10361-1-AP

    IF Staining of HeLa using 10361-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BNIP2 antibody (10361-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IF Staining of RAW 264.7 using 10361-1-AP

    IF Staining of RAW 264.7 using 10361-1-AP

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using BNIP2 antibody (10361-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

BNIP2 Polyclonal antibody

BNIP2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 10361-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

NIP2, BNIP 2, BCL2/adenovirus E1B 19 kDa protein-interacting protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHCT 116 cells, MCF-7 cells, MDA-MB-231 cells, SW480 cells
Positive IF/ICC detected inHeLa cells, RAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy BNIP2 Ready-To-Use IHC Kit. BNIP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10361-1-AP targets BNIP2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0403

Product name: Recombinant human BNIP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 144-313 aa of BC002461

Sequence: MKAIEPYKKVISHGGYYGDGLNAIVVFAVCFMPESSQPNYRYLMDNLFKYVIGTLELLVAENYMIVYLNGATTRRKMPSLGWLRKCYQQIDRRLRKNLKSLIIVHPSWFIRTLLAVTRPFISSKFSQKIRYVFNLAELAELVPMEYVGIPECIKQVDQELNGKQDEPKNE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BCL2/adenovirus E1B 19kDa interacting protein 2
别名 NIP2, BNIP 2, BCL2/adenovirus E1B 19 kDa protein-interacting protein 2
计算分子量 36 kDa
观测分子量 43 kDa
GenBank蛋白编号BC002461
基因名称 BNIP2
Gene ID (NCBI) 663
RRIDAB_3085366
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12982
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL2/adenovirus E1B 19 kDa protein-interacting protein 2 (BNIP2) is also named as PRKACN2. BNIP2 is developmentally regulated may suggest a role of this gene in those brain areas where the differentiation is orchestrated by estradiol. The expression of BNIP2 was inhibited by estradiol. BNIP2 is implicated in the suppression of cell death and interacts with the BCL-2 and adenovirus E1B 19 kDa proteins. BNIP2 is highly expressed in patients with favorable neuroblastoma (NB) (PMID: 25611382).

实验方案

Product Specific Protocols
IF protocol for BNIP2 antibody 10361-1-APDownload protocol
WB protocol for BNIP2 antibody 10361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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