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BNIP2 Polyclonal antibody

BNIP2 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10361-1-AP

Print datasheet

Synonyms

BNIP 2, BNIP2, NIP2

验证数据展示

  • WB analysis using 10361-1-AP

    WB analysis using 10361-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10361-1-AP (BNIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 10361-1-AP

    IF Staining of HeLa using 10361-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BNIP2 antibody (10361-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IF Staining of RAW 264.7 using 10361-1-AP

    IF Staining of RAW 264.7 using 10361-1-AP

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using BNIP2 antibody (10361-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells, MDA-MB-231 cells, SW480 cells
Positive IF detected inHeLa cells, RAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10361-1-AP targets BNIP2 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human, mouse
Immunogen BNIP2 fusion protein Ag0403 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BCL2/adenovirus E1B 19kDa interacting protein 2
Synonyms BNIP 2, BNIP2, NIP2
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC002461
Gene Symbol BNIP2
Gene ID (NCBI) 663
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12982
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL2/adenovirus E1B 19 kDa protein-interacting protein 2 (BNIP2) is also named as PRKACN2. BNIP2 is developmentally regulated may suggest a role of this gene in those brain areas where the differentiation is orchestrated by estradiol. The expression of BNIP2 was inhibited by estradiol. BNIP2 is implicated in the suppression of cell death and interacts with the BCL-2 and adenovirus E1B 19 kDa proteins. BNIP2 is highly expressed in patients with favorable neuroblastoma (NB) (PMID: 25611382).

实验方案

Product Specific Protocols
WB protocol for BNIP2 antibody 10361-1-APDownload protocol
IF protocol for BNIP2 antibody 10361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols