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验证数据展示

  • WB analysis using 11564-1-AP (same clone as 11564-1-PBS)

    WB analysis using 11564-1-AP (same clone as 11564-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11564-1-AP (BMSC UbP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11564-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 11564-1-AP (same clone as 11564-1-PBS)

    IP experiment of HeLa using 11564-1-AP (same clone as 11564-1-PBS)

    IP result of anti-BMSC UbP (IP:11564-1-AP, 4ug; Detection:11564-1-AP 1:1000) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 11564-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 11564-1-AP (same clone as 11564-1-PBS)

    IHC staining of human gliomas using 11564-1-AP (same clone as 11564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11564-1-AP (BMSC UbP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11564-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 11564-1-AP (same clone as 11564-1-PBS)

    IF Staining of HepG2 using 11564-1-AP (same clone as 11564-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 11564-1-AP (BMSC UbP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11564-1-PBS in a different storage buffer formulation.

BMSC UbP Polyclonal antibody, PBS Only

BMSC UbP Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11564-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

BMSCUBP, BMSC-UbP, Bone marrow stromal cell ubiquitin-like protein, SB132, Ubiquitin-like protein 7

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11564-1-PBS targets BMSC UbP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2130

Product name: Recombinant human UBL7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-380 aa of BC030055

Sequence: MSLSDWHLAVKLADQPLTPKSILRLPETELGEYSLGGYSISFLKQLIAGKLQESVPDPELIDLIYCGRKLKDDQTLDFYGIQPGSTVHVLRKSWPEPDQKPEPVDKVAAMREFRVLHTALHSSSSYREAVFKMLSNKESLDQIIVATPGLSSDPIALGVLQDKDLFSVFADPNMLDTLVPAHPALVNAIVLVLHSVAGSAPMPGTDSSSRSMPSSSYRDMPGGFLFEGLSDDEDDFHPNTRSTPSSSTPSSRPASLGYSGAAGPRPITQSELATALALASTPESSSHTPTPGTQGHSSGTSPMSSGVQSGTPITNDLFSQALQHALQASGQPSLQSQWQPQLQQLRDMGIQDDELSLRALQATGGDIQAALELIFAGGAP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin-like 7 (bone marrow stromal cell-derived)
别名 BMSCUBP, BMSC-UbP, Bone marrow stromal cell ubiquitin-like protein, SB132, Ubiquitin-like protein 7
计算分子量 380 aa, 41 kDa
观测分子量 41 kDa
GenBank蛋白编号BC030055
基因名称 UBL7
Gene ID (NCBI) 84993
RRIDAB_2212080
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96S82
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ubiquitin is an important cellular signal that targets proteins for degradation or regulates their functions. UBL7 (ORF name SB132) encodes ubiquitin-like protein7 also named bone marrow stromal cell ubiquitin-like protein (BMSC-UbP) capable of binding ubiquitin. BMSC-UbP protein is derived from bone marrow stromal cells containing a ubiquitin-associated (UBA) domain at the C terminus that has been implicated in linking cellular processes and the ubiquitin system. BMSC-UbP mRNA is widely expressed in human multiple tissues and various tumor cell lines and is decreased in BMSC stimulated with phorbol myristate acetate (PMA) suggesting a role in regulation of BMSC function.

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