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验证数据展示

  • WB analysis using 82928-1-RR

    WB analysis using 82928-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82928-1-RR (BMPR1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 82928-1-RR

    FC experiment of MCF-7 using 82928-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug BMPR1A Recombinant antibody (82928-1-RR, Clone:230137B7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IP experiment of HEK-293 using 82928-1-RR

    IP experiment of HEK-293 using 82928-1-RR

    IP result of anti-BMPR1A (IP:82928-1-RR, 4ug; Detection:82928-1-RR 1:2000) with HEK-293 cells lysate 1920 ug.

BMPR1A Recombinant monoclonal antibody

BMPR1A Uni-rAb® Recombinant Antibody for WB, FC (Intra), IP, ELISA
Cat No. 82928-1-RR

产品说明书

CloneNo. 230137B7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), IP, ELISA

别名

230137B7, Activin receptor like kinase 3, Activin receptor-like kinase 3, ACVRLK3, ALK 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, U2OS cells, HEK-293 cells, mouse heart tissue
Positive IP detected inHEK-293 cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82928-1-RR targets BMPR1A in WB, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3410

Product name: Recombinant human BMPR1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 174-427 aa of BC028383

Sequence: FCYKHYCKSISSRRRYNRDLEQDEAFIPVGESLKDLIDQSQSSGSGSGLPLLVQRTIAKQIQMVRQVGKGRYGEVWMGKWRGEKVAVKVFFTTEEASWFRETEIYQTVLMRHENILGFIAADIKGTGSWTQLYLITDYHENGSLYDFLKCATLDTRALLKLAYSAACGLCHLHTEIYGTQGKPAIAHRDLKSKNILIKKNGSCCIADLGLAVKFNSDTNEVDVPLNTRVGTKRYMAPEVLDESLNKNHFQPYIM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 bone morphogenetic protein receptor, type IA
别名 230137B7, Activin receptor like kinase 3, Activin receptor-like kinase 3, ACVRLK3, ALK 3
计算分子量 532 aa, 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC028383
基因名称 BMPR1A
Gene ID (NCBI) 657
RRIDAB_3670667
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP36894
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BMPR1A (Bone morphogenetic protein receptor type-1A) is also named as ACVRLK3, ALK3 and SKR5. BMPR1A is an essential receptor for BMP signaling (PMID: 26279380). BMPR1A is necessary for chondrogenesis and osteogenesis (PMID: 32764110). BMPR1A is a cell surface marker of human SuSCs (PMID: 33658353).

实验方案

Product Specific Protocols
IP protocol for BMPR1A antibody 82928-1-RRDownload protocol
WB protocol for BMPR1A antibody 82928-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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