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验证数据展示

  • IHC staining of human skin squamous cell cancer using 12702-1-AP

    IHC staining of human skin squamous cell cancer using 12702-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin squamous cell cancer tissue slide using 12702-1-AP (BMPR1A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin squamous cell cancer using 12702-1-AP

    IHC staining of human skin squamous cell cancer using 12702-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin squamous cell cancer tissue slide using 12702-1-AP (BMPR1A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 12702-1-AP

    IHC staining of human colon cancer using 12702-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12702-1-AP (BMPR1A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 12702-1-AP

    IHC staining of human colon cancer using 12702-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12702-1-AP (BMPR1A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 12702-1-AP

    IF Staining of HeLa using 12702-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BMPR1A antibody (12702-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of U2OS using 12702-1-AP

    IF Staining of U2OS using 12702-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using BMPR1A antibody (12702-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

BMPR1A Polyclonal antibody

BMPR1A Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 12702-1-AP

产品说明书

引用文献(20)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

IHC, IF/ICC, ELISA and More (3)

别名

Activin receptor like kinase 3, Activin receptor-like kinase 3, ACVRLK3, ALK 3, ALK3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman skin squamous cell cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, U2OS cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12702-1-AP targets BMPR1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag3410

Product name: Recombinant human BMPR1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 174-427 aa of BC028383

Sequence: FCYKHYCKSISSRRRYNRDLEQDEAFIPVGESLKDLIDQSQSSGSGSGLPLLVQRTIAKQIQMVRQVGKGRYGEVWMGKWRGEKVAVKVFFTTEEASWFRETEIYQTVLMRHENILGFIAADIKGTGSWTQLYLITDYHENGSLYDFLKCATLDTRALLKLAYSAACGLCHLHTEIYGTQGKPAIAHRDLKSKNILIKKNGSCCIADLGLAVKFNSDTNEVDVPLNTRVGTKRYMAPEVLDESLNKNHFQPYIM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bone morphogenetic protein receptor, type IA
别名 Activin receptor like kinase 3, Activin receptor-like kinase 3, ACVRLK3, ALK 3, ALK3
计算分子量 532 aa, 60 kDa
观测分子量 60-68 kDa
GenBank蛋白编号BC028383
基因名称 BMPR1A
Gene ID (NCBI) 657
RRIDAB_2064273
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP36894
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for BMPR1A antibody 12702-1-APDownload protocol
IHC protocol for BMPR1A antibody 12702-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

EBioMedicine

H3K27me3-modulated Hofbauer cell BMP2 signalling enhancement compensates for shallow trophoblast invasion in preeclampsia

Authors - Jianye Deng
View Article
mouseIHC

Proc Natl Acad Sci U S A

Uterine ALK3 is essential during the window of implantation

Authors - Diana Monsivais
View Article
ratWB

Arterioscler Thromb Vasc Biol

Binding of Hypoxia-Induced Mitogenic Factor/RELM-β to Bone Morphogenetic Protein Receptor 2 Complex Promotes Pulmonary Hypertension.

Authors - Ting Zhang
  • KO Validated
View Article
humanWB

ACS Appl Mater Interfaces

Valproic Acid-Loaded ZIF-8 Nanoparticles with Antibacterial, Immunomodulatory, and Dentin Regenerative Functions for Pulpitis Therapy.

Authors - Yufan Wei
View Article

Cell Rep

Lineage tracing reveals a shared cellular origin for supraclavicular brown and inguinal beige adipocytes.

Authors - Yali Ran
View Article
mouseWB

Biomed Pharmacother

Pilose antler extract promotes hair growth in androgenic alopecia mice by promoting the initial anagen phase

Authors - Menghua Liu
View Article
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