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验证数据展示

  • WB analysis using 12221-1-AP (same clone as 12221-1-PBS)

    WB analysis using 12221-1-AP (same clone as 12221-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12221-1-AP (BMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12221-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 12221-1-AP (same clone as 12221-1-PBS)

    WB analysis of HEK-293 using 12221-1-AP (same clone as 12221-1-PBS)

    WB result of BMP7 antibody (12221-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BMP7 transfected HEK-293 cells. This data was developed using the same antibody clone with 12221-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 12221-1-AP (same clone as 12221-1-PBS)

    IHC staining of human kidney using 12221-1-AP (same clone as 12221-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12221-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 12221-1-AP (same clone as 12221-1-PBS)

    IHC staining of human kidney using 12221-1-AP (same clone as 12221-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12221-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 12221-1-AP (same clone as 12221-1-PBS)

    IP experiment of HEK-293 using 12221-1-AP (same clone as 12221-1-PBS)

    IP result of anti-BMP7 (IP:12221-1-AP, 4ug; Detection:12221-1-AP 1:800) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 12221-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12221-1-AP (same clone as 12221-1-PBS)

    IF Staining of HeLa using 12221-1-AP (same clone as 12221-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BMP7 antibody (12221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12221-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

BMP7 Polyclonal antibody, PBS Only

BMP7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12221-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

OP1, OP 1, Eptotermin alfa, Bone morphogenetic protein 7, BMP-7

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12221-1-PBS targets BMP7 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2902

Product name: Recombinant human BMP7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 82-431 aa of BC008584

Sequence: FMLDLYNAMAVEEGGGPGGQGFSYPYKAVFSTQGPPLASLQDSHFLTDADMVMSFVNLVEHDKEFFHPRYHHREFRFDLSKIPEGEAVTAAEFRIYKDYIRERFDNETFRISVYQVLQEHLGRESDLFLLDSRTLWASEEGWLVFDITATSNHWVVNPRHNLGLQLSVETLDGQSINPKLAGLIGRHGPQNKQPFMVAFFKATEVHFRSIRSTGSKQRSQNRSKTPKNQEALRMANVAENSSSDQRQACKKHELYVSFRDLGWQDWIIAPEGYAAYYCEGECAFPLNSYMNATNHAIVQTLVHFINPETVPKPCCAPTQLNAISVLYFDDSSNVILKKYRNMVVRACGCH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bone morphogenetic protein 7
别名 OP1, OP 1, Eptotermin alfa, Bone morphogenetic protein 7, BMP-7
计算分子量 431 aa, 49 kDa
观测分子量 43-49 kDa
GenBank蛋白编号BC008584
基因名称 BMP7
Gene ID (NCBI) 655
RRIDAB_2063960
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP18075
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site.

BMP7, also known as osteogenic protein-1 or OP-1, plays a key role in the transformation of mesenchymal cells into bone and cartilage. BMP7 may be involved in bone homeostasis (PMID: 15621726). It is expressed in the brain, kidneys and bladder. BMP7 is also present in cancers, including breast, prostate, and colon cancers, in which it is implicated in regulating cancer cell proliferation (PMID: 16419056, PMID: 15531927). Overexpression of BMP7 mRNA in colorectal cancer patients was significantly associated with poor prognosis and low overall survival (PMID: 18259822). Recent studies suggest that high-expression level of BMP7 serves as a biomarker for poor prognosis for HCC (PMID: 23179403).

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