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验证数据展示

  • IF Staining of A549 using 68175-1-Ig (same clone as 68175-1-PBS)

    IF Staining of A549 using 68175-1-Ig (same clone as 68175-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using BLVRA antibody (68175-1-Ig, Clone: 2A10F2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68175-1-PBS in a different storage buffer formulation.

  • WB analysis using 68175-1-Ig (same clone as 68175-1-PBS)

    WB analysis using 68175-1-Ig (same clone as 68175-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68175-1-Ig (BLVRA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68175-1-PBS in a different storage buffer formulation.

BLVRA Monoclonal antibody, PBS Only

BLVRA Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68175-1-PBS

产品说明书

CloneNo. 2A10F2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

BVR A, BVR, BLVR, Biliverdin-IX alpha-reductase, biliverdin reductase A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68175-1-PBS targets BLVRA in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag32730

Product name: Recombinant human BLVRA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-296 aa of BC008456

Sequence: MNAEPERKFGVVVVGVGRAGSVRMRDLRNPHPSSAFLNLIGFVSRRELGSIDGVQQISLEDALSSQEVEVAYICSESSSHEDYIRQFLNAGKHVLVEYPMTLSLAAAQELWELAEQKGKVLHEEHVELLMEEFAFLKKEVVGKDLLKGSLLFTAGPLEEERFGFPAFSGISRLTWLVSLFGELSLVSATLEERKEDQYMKMTVCLETEKKSPLSWIEEKGPGLKRNRYLSFHFKSGSLENVPNVGVNKNIFLKDQNIFVQKLLGQFSEKELAAEKKRILHCLGLAEEIQKYCCSRK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 biliverdin reductase A
别名 BVR A, BVR, BLVR, Biliverdin-IX alpha-reductase, biliverdin reductase A
计算分子量33.2 kDa
观测分子量 37-40 kDa, 66 kDa
GenBank蛋白编号BC008456
基因名称 BLVRA
Gene ID (NCBI) 644
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP53004
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BLVRA(Biliverdin reductase A) is also named BLVR, BVR, and belongs to the biliverdin reductase subfamily. It catalyzes the conversion of biliverdin to bilirubin in the presence of NADPH or NADH. This protein can exist as a dimer(PMID:11773068). Defects in BLVRA are the cause of hyperbiliverdinemia (HBLVD).

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