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  • Featured Product
  • KD/KO Validated

BLVRA Polyclonal antibody

BLVRA Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10775-1-AP

Print datasheet

Synonyms

Biliverdin IX alpha reductase, biliverdin reductase A, BLVR, BLVRA, BVR, BVR A, BVRA

验证数据展示

  • WB analysis using 10775-1-AP

    WB analysis using 10775-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10775-1-AP (BLVRA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 10775-1-AP

    IHC staining of human prostate cancer using 10775-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10775-1-AP (BLVRA antibody) at dilution of 1:100 (under 10x lens).

  • IF Staining of L02 using 10775-1-AP

    IF Staining of L02 using 10775-1-AP

    Immunofluorescent analysis of L02 cells using 10775-1-AP (BLVRA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, L02 cells, mouse liver tissue, HepG2 cells, HuH-7 cells, PC-3 cells, SH-SY5Y cells
Positive IHC detected inhuman prostate cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inL02 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10775-1-AP targets BLVRA in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen BLVRA fusion protein Ag1166 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name biliverdin reductase A
Synonyms Biliverdin IX alpha reductase, biliverdin reductase A, BLVR, BLVRA, BVR, BVR A, BVRA
Calculated Molecular Weight33.2 kDa
Observed Molecular Weight 37-40 kDa, 66 kDa
GenBank Accession NumberBC008456
Gene Symbol BLVRA
Gene ID (NCBI) 644
RRIDAB_2065073
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP53004
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BLVRA(Biliverdin reductase A) is also named as BLVR, BVR and belongs to the biliverdin reductase subfamily.It catalyze the conversion of biliverdin to bilirubin in the presence of NADPH or NADH.This protein can exsit as a dimer(PMID:11773068). Defects in BLVRA are the cause of hyperbiliverdinemia (HBLVD).

实验方案

Product Specific Protocols
WB protocol for BLVRA antibody 10775-1-APDownload protocol
IHC protocol for BLVRA antibody 10775-1-APDownload protocol
IF protocol for BLVRA antibody 10775-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cancer Manag Res

Biliverdin Reductase A (BLVRA) Promotes Colorectal Cancer Cell Progression by Activating the Wnt/β-Catenin Signaling Pathway.

Authors - Haiyan Mao
  • KD Validated
View Article
humanIF

Arch Biochem Biophys

Biliverdin reductase deficiency triggers an endothelial-to-mesenchymal transition in human endothelial cells.

Authors - Damian Klóska
View Article
humanWB

EXCLI J

Induction of cell apoptosis by biliverdin reductase inhibitor in MCF-7 and MDA-MB-468 breast cancer cell lines: Experimental and in silico studies.

Authors - Seyedeh Zahra Shahrokhi
View Article