验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12165-1-AP targets BLOC1S2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag2807 Product name: Recombinant human BLOC1S2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-99 aa of BC020494 Sequence: MFSKMATYLTGELTATSEDYKLLENMNKLTSLKYLEMKDIAINISRNLKDLNQKYAGLQPYLDQINVIEEQVAALEQAAYKLDAYSKKLEAKYKKLEKR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | biogenesis of lysosomal organelles complex-1, subunit 2 |
| 别名 | Biogenesis of lysosome-related organelles complex 1 subunit 2, BLOC 1 subunit 2, BLOC-1 subunit 2, BLOS2, CEAP |
| 计算分子量 | 142 aa, 16 kDa |
| 观测分子量 | 17 kDa |
| GenBank蛋白编号 | BC020494 |
| 基因名称 | BLOC1S2 |
| Gene ID (NCBI) | 282991 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6QNY1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BLOS2, also named BLOC1S2, is a shared subunit of two lysosomal trafficking complexes, biogenesis of lysosome-related organelles complex-1 (BLOC-1) and BLOC-1 related complex. Loss of BLOS2 resulted in elevated Notch signaling which consequently increased the proliferation of neural progenitor cells and inhibited neuronal differentiation in cortices. BLOS2 is a novel negative player in regulating Notch signaling through lysosomal trafficking by controlling multiple stem and progenitor cell homeostasis in vertebrates. (PMID 27719760)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BLOC1S2 antibody 12165-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
