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验证数据展示

  • WB analysis using 30254-1-AP (same clone as 30254-1-PBS)

    WB analysis using 30254-1-AP (same clone as 30254-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30254-1-AP (BLM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30254-1-PBS in a different storage buffer formulation.

  • WB analysis using 30254-1-AP (same clone as 30254-1-PBS)

    WB analysis using 30254-1-AP (same clone as 30254-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30254-1-AP (BLM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30254-1-PBS in a different storage buffer formulation.

BLM Polyclonal antibody, PBS Only

BLM Polyclonal Antibody for WB, Indirect ELISA
Cat No. 30254-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

RECQL3, RECQL2, RECQ2, BS

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30254-1-PBS targets BLM in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32996

Product name: Recombinant human BLM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1235-1417 aa of

Sequence: VHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Bloom syndrome, RecQ helicase-like
别名 RECQL3, RECQL2, RECQ2, BS
计算分子量159 kDa
观测分子量160-175 kDa
基因名称 BLM
Gene ID (NCBI) 641
RRIDAB_3086282
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54132
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BLM participates in DNA replication and repair, it is involved in 5'-end resection of DNA during double-strand break (DSB) repair. BLM can also be recruited by the KHDC3L-OOEP scaffold to DNA replication forks where it is retained by TRIM25 ubiquitination, it thereby promotes the restart of stalled replication forks.

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