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验证数据展示

  • WB analysis using 84625-3-RR (same clone as 84625-3-PBS)

    WB analysis using 84625-3-RR (same clone as 84625-3-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 84625-3-RR (BLIMP1/PRDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84625-3-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 84625-3-RR (same clone as 84625-3-PBS)

    IF Staining of A549 using 84625-3-RR (same clone as 84625-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using BLIMP1/PRDM1 antibody (84625-3-RR, Clone: 242030B1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84625-3-PBS in a different storage buffer formulation.

BLIMP1/PRDM1 Recombinant monoclonal antibody, PBS Only

BLIMP1/PRDM1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84625-3-PBS

产品说明书

CloneNo. 242030B1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

BLIMP1, PRDM1, 242030B1, BLIMP 1, PRDI BF1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84625-3-PBS targets BLIMP1/PRDM1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg2625

Product name: Recombinant Human PRDM1 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 45-230 aa of NM_001198.3

Sequence: DMTLWTEAEFEEKCTYIVNDHPWDSGADGGTSVQAEASLPRNLLFKYATNSEEVIGVMSKEYIPKGTRFGPLIGEIYTNDTVPKNANRKYFWRIYSRGELHHFIDGFNEEKSNWMRYVNPAHSPREQNLAACQNGMNIYFYTIKPIPANQELLVWYCRDFAERLHYPYPGELTMMNLTQTQSSLKQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PR domain containing 1, with ZNF domain
别名 BLIMP1, PRDM1, 242030B1, BLIMP 1, PRDI BF1
计算分子量92 kDa
观测分子量 96-110 kDa
GenBank蛋白编号NM_001198.3
基因名称 PRDM1
Gene ID (NCBI) 639
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO75626-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BLIMP1(B lymphocyte-induced maturation protein 1), also known as PRDM1(PR domain zinc finger protein 1), is an evolutionarily conserved transcriptional regulator originally described as a repressor of gene transcription(PMID: 35154079). BLIMP1/PRDM1 is widely expressed in multiple cell types including B lymphocytes, T lymphocytes, mammary luminal progenitor cells and skin epithelial cells. BLIMP1/PRDM1 is a master regulator of terminal B cell differentiation and may also function as a tumor suppressor in the pathogenesis of DLBCL, where it is frequently inactivated by mutations and deletions.

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