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  • KD/KO Validated

cIAP1 Polyclonal antibody

cIAP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10022-1-AP

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Synonyms

API1, BIRC2, C IAP1, cIAP1, Hiap 2, HIAP2, IAP 2, IAP homolog B, IAP2, MIHB, RING finger protein 48, RNF48



经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, Jurkat cells, mouse brain tissue, mouse liver tissue, mouse testis tissue
Positive IHC detected inhuman testis tissue, mouse pancreas tissue, mouse testis tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy BIRC2/cIAP1 Ready-To-Use IHC Kit. BIRC2/cIAP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10022-1-AP targets cIAP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, zebrafish
Immunogen cIAP1 fusion protein Ag15203 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name baculoviral IAP repeat-containing 2
Synonyms API1, BIRC2, C IAP1, cIAP1, Hiap 2, HIAP2, IAP 2, IAP homolog B, IAP2, MIHB, RING finger protein 48, RNF48
Calculated Molecular Weight 618 aa, 70 kDa
Observed Molecular Weight 70 kDa, 55-60 kDa
GenBank Accession NumberBC016174
Gene Symbol BIRC2
Gene ID (NCBI) 329
RRIDAB_2063790
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13490
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BIRC2 (also known as cIAP1) is a member of the inhibitor of apoptosis protein (IAP) family. The inhibitor of apoptosis (IAP) proteins are a family of anti-apoptotic regulators found in viruses and metazoans. BIRC2 is a nuclear shuttling protein, whose subcellular localization is mediated by the CRM1-dependent nuclear export pathway (PMID: 15265700). The protein is regulated transcriptionally and can be inhibited by mitochondrial proteins released in the cytoplasm upon apoptotic stimuli (PMID: 15187025). BIRC2 is also believed to be a critical regulator of vascular integrity and endothelial cell survival, thereby providing an additional target pathway for the control of angiogenesis and blood vessel homeostasis during embryogenesis, regeneration and tumorigenesis (PMID: 17934460). This BIRC2 antibody (10022-1-AP) can bind both full length (70kd) and cleaved form (60kd) of the protein.

实验方案

Product Specific Protocols
WB protocol for cIAP1 antibody 10022-1-APDownload protocol
IHC protocol for cIAP1 antibody 10022-1-APDownload protocol
IF protocol for cIAP1 antibody 10022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
zebrafishWB,IF

Nat Genet

Birc2 (cIap1) regulates endothelial cell integrity and blood vessel homeostasis.

Authors - Santoro Massimo M MM
  • KD Validated
humanIF

Mol Cell

Liquid phase separation of NEMO induced by polyubiquitin chains activates NF-κB.

Authors - Mingjian Du
humanWB

Mol Cell

A cytoplasmic ATM-TRAF6-cIAP1 module links nuclear DNA damage signaling to ubiquitin-mediated NF-κB activation.

Authors - Michael Hinz
  • KD Validated
mouseWB

Acta Pharm Sin B

A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein.

Authors - Ziqiao Yuan
humanWB

Dev Cell

Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform.

Authors - Maria Zachari
mouseWB

J Immunol

Differential TRAF3 utilization by a variant human CD40 receptor with enhanced signaling.

Authors - Peters Anna L AL