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验证数据展示

  • WB analysis using 14245-1-AP (same clone as 14245-1-PBS)

    WB analysis using 14245-1-AP (same clone as 14245-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14245-1-AP (BIN2 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

  • IHC staining of human spleen using 14245-1-AP (same clone as 14245-1-PBS)

    IHC staining of human spleen using 14245-1-AP (same clone as 14245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

  • IHC staining of human spleen using 14245-1-AP (same clone as 14245-1-PBS)

    IHC staining of human spleen using 14245-1-AP (same clone as 14245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 14245-1-AP (same clone as 14245-1-PBS)

    IP experiment of Jurkat using 14245-1-AP (same clone as 14245-1-PBS)

    IP result of anti-BIN2 (IP:14245-1-AP, 4ug; Detection:14245-1-AP 1:400) with Jurkat cells lysate 2000ug. This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

BIN2 Polyclonal antibody, PBS Only

BIN2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 14245-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

14245-1-PBS targets BIN2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag5502

Product name: Recombinant human BIN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 216-565 aa of BC047686

Sequence: LRDVFYREMSKLNHNLYEVMSKLEKQHSNKVFVVKGLSSSSRRSLVISPPVRTATVSSPLTSPTSPSTLSLKSESESVSATEDLAPDAAQGEDNSEIKELLEEEEIEKEGSEASSSEEDEPLPACNGPAQAQPSPTTERAKSQEEVLPSSTTPSPGGALSPSGQPSSSATEVVLRTRTASEGSEQPKKRASIQRTSAPPSRPPPPRATASPRPSSGNIPSSPTASGGGSPTSPRASLGTGTASPRTSLEVSPNPEPPEKPVRTPEAKENENIHNQNPEELCTSPTLMTSQVASEPGEAKKMEDKEKDNKLISADSSEGQDQLQVSMVPENNNLTAPEPQEEVSTSENPQL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bridging integrator 2
别名
计算分子量 62 kDa
观测分子量 80-90 kDa
GenBank蛋白编号BC047686
基因名称 BIN2
Gene ID (NCBI) 51411
RRIDAB_2063647
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UBW5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BAR proteins, which are characterized by a common N-terminal BAR domain, are a family of adaptor proteins implicated in a diverse set of cellular processes. BIN1 (bridging integrator 1) and BIN2 are BAR proteins. The human BIN2 gene is located at chromosome 4q22.1, and encodes a 565-amino acid protein with a predicted molecular weight of ~62 kDa. It shows preferential expression in hematopoietic tissues, with highest mRNA levels in spleen and peripheral blood leukocytes and also high levels in thymus, colon, and placenta. BIN2 has been shown to migrate with an apparent mobility of ~80 kDa, and interact with BIN1. (PMID: 10903846)

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