验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, Hela cells, Jurkat cells, K-562 cells, MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
29603-1-AP targets BICD2 in WB, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | Human |
免疫原 | BICD2 fusion protein Ag31279 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | bicaudal D homolog 2 (Drosophila) |
别名 | bA526D8.1, Bic D 2, BICD2, KIAA0699, Protein bicaudal D homolog 2 |
计算分子量 | 855 aa, 97 kDa |
观测分子量 | 93-96 kDa |
GenBank蛋白编号 | BC073970 |
基因名称 | BICD2 |
Gene ID (NCBI) | 23299 |
RRID | AB_2935474 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8TD16 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein bicaudal D homolog 2 (BICD2) acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates and stabilizes the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Facilitates the binding of RAB6A to the Golgi by stabilizing its GTP-bound form. Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport via its interaction with RAB6A and recruitment of the dynein-dynactin motor complex (PubMed:25962623).
实验方案
Product Specific Protocols | |
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WB protocol for BICD2 antibody 29603-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |