验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16351-1-AP targets BHMT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag9558 Product name: Recombinant human BHMT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-363 aa of BC020665 Sequence: MAPAGRPGAKKGILERLESGEVVIGDGSFLITLEKRGYVKAGLWTPEAVIEHPDAVRQLHMEFLRAGSNVMQTFTFSASEDNMESKWEDVNAAACDLAREVAGKGDALVAGGICQTSIYKYQKDEARIKKLFRQQLEVFAWKNVDFLIAEYFEHVEEAVWAVEVLKESDRPVAVTMCIGPEGDMHDITPGECAVRLVKAGASIVGVNCRFGPDTSLKTMELMKEGLEWAGLKAHLMVQPLGFHAPDCGKEGFVDLPEYPFGLESRVATRWDIQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWIRARARREYWENLLPASGRPFCPSLSKPDF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | betaine-homocysteine methyltransferase 2 |
| 别名 | betaine homocysteine methyltransferase 2, Betaine--homocysteine S-methyltransferase 2, EC:2.1.1.10, S-methylmethionine--homocysteine S-methyltransferase BHMT2, SMM-hcy methyltransferase |
| 计算分子量 | 363 aa, 40 kDa |
| 观测分子量 | 40-45 kDa |
| GenBank蛋白编号 | BC020665 |
| 基因名称 | BHMT2 |
| Gene ID (NCBI) | 23743 |
| RRID | AB_2878248 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H2M3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BHMT2 (Betaine-Homocysteine S-Methyltransferase 2) is a zinc-dependent metalloenzyme that plays a crucial role in regulating amino acid homeostasis and cellular methylation pathways. BHMT2 specifically utilizes S-methylmethionine (SMM)-often called Vitamin U, derived from plant-based foods-as its methyl donor. BHMT2 is most heavily expressed in tissues with strong metabolic activity, particularly the liver and kidneys.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for BHMT2 antibody 16351-1-AP | Download protocol |
| WB protocol for BHMT2 antibody 16351-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
