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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 86372-1-RR (same clone as 86372-1-PBS)

    WB analysis using 86372-1-RR (same clone as 86372-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86372-1-RR (BHMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86372-1-PBS in a different storage buffer formulation.

  • IP experiment of rat liver using 86372-1-RR (same clone as 86372-1-PBS)

    IP experiment of rat liver using 86372-1-RR (same clone as 86372-1-PBS)

    IP result of anti-BHMT (IP:86372-1-RR, 4ug; Detection:86372-1-RR 1:3000) with rat liver tissue lysate 3080 ug. This data was developed using the same antibody clone with 86372-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86372-1-RR

    Affinity and Kinetic Characterization of 86372-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86372-1-RR against Human BHMT were performed. The affinity constant is below 1 pM.

    {{ptg:ProductFeaturesInfo}}

BHMT Recombinant monoclonal antibody, PBS Only

BHMT Uni-rAb® Recombinant Antibody for WB, IP, Indirect ELISA
Cat No. 86372-1-PBS

产品说明书

CloneNo. 250978E3

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IP, Indirect ELISA

别名

Betaine--homocysteine S-methyltransferase 1, EC:2.1.1.5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BHMT Ready-To-Use IHC Kit. BHMT primary antibody included.

产品信息

86372-1-PBS targets BHMT in WB, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag8738

Product name: Recombinant human BHMT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-406 aa of BC012616

Sequence: MPPVGGKKAKKGILERLNAGEIVIGDGGFVFALEKRGYVKAGPWTPEAAVEHPEAVRQLHREFLRAGSNVMQTFTFYASEDKLENRGNYVLEKISGQEVNEAACDIARQVADEGDALVAGGVSQTPSYLSCKSETEVKKVFLQQLEVFMKKNVDFLIAEYFEHVEEAVWAVETLIASGKPVAATMCIGPEGDLHGVPPGECAVRLVKAGASIIGVNCHFDPTISLKTVKLMKEGLEAARLKAHLMSQPLAYHTPDCNKQGFIDLPEFPFGLEPRVATRWDIQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWVRARARKEYWENLRIASGRPYNPSMSKPDGWGVTKGTAELMQQKEATTEQQLKELFEKQKFKSQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 betaine-homocysteine methyltransferase
别名 Betaine--homocysteine S-methyltransferase 1, EC:2.1.1.5
计算分子量 406 aa, 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC012616
基因名称 BHMT
Gene ID (NCBI) 635
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ93088
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Betaine-homocysteine methyltransferase (BHMT) is a cytosolic enzyme that Involved in the regulation of homocysteine metabolism. BHMT catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline(PMID: 8798461, 10529246).