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验证数据展示

  • WB analysis using 17895-1-AP

    WB analysis using 17895-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17895-1-AP (BHLHE40 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 17895-1-AP

    IP experiment of HeLa using 17895-1-AP

    IP result of anti-BHLHE40 (IP:17895-1-AP, 4ug; Detection:17895-1-AP 1:1000) with HeLa cells lysate 1800 ug.

  • IHC staining of human endometrial cancer using 17895-1-AP

    IHC staining of human endometrial cancer using 17895-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 17895-1-AP (BHLHE40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human endometrial cancer using 17895-1-AP

    IHC staining of human endometrial cancer using 17895-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 17895-1-AP (BHLHE40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

BHLHE40 Polyclonal antibody

BHLHE40 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 17895-1-AP

产品说明书

引用文献(25)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IP, ELISA and More (2)

别名

DEC1, bHLHb2, Class B basic helix-loop-helix protein 2, Class E basic helix-loop-helix protein 40, Enhancer-of-split and hairy-related protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, C2C12 cells, HeLa cells, MDA-MB-231 cells, L02 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman endometrial cancer tissue, human pancreas cancer tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17895-1-AP targets BHLHE40 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, ChIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, bovine
免疫原

CatNo: Ag12236

Product name: Recombinant human BHLHE40 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-412 aa of BC082238

Sequence: MERIPSAQPPPACLPKAPGLEHGDLPGMYPAHMYQVYKSRRGIKRSEDSKETYKLPHRLIEKKRRDRINECIAQLKDLLPEHLKLTTLGHLEKAVVLELTLKHVKALTNLIDQQQQKIIALQSGLQAGELSGRNVETGQEMFCSGFQTCAREVLQYLAKHENTRDLKSSQLVTHLHRVVSELLQGGTSRKPSDPAPKVMDFKEKPSSPAKGSEGPGKNCVPVIQRTFAHSSGEQSGSDTDTDSGYGGESEKGDLRSEQPCFKSDHGRRFTMGERIGAIKQESEEPPTKKNRMQLSDDEGHFTSSDLISSPFLGPHPHQPPFCLPFYLIPPSATAYLPMLEKCWYPTSVPVLYPGLNASAAALSSFMNPDKISAPLLMPQRLPSPLPAHPSVDSSVLLQALKPIPPLNLETKD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 basic helix-loop-helix family, member e40
别名 DEC1, bHLHb2, Class B basic helix-loop-helix protein 2, Class E basic helix-loop-helix protein 40, Enhancer-of-split and hairy-related protein 2
计算分子量 412 aa, 46 kDa
观测分子量 46-50 kDa
GenBank蛋白编号BC082238
基因名称 BHLHE40
Gene ID (NCBI) 8553
RRIDAB_2065351
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14503
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BHLHE40 (Basic Helix-Loop-Helix Family Member E40), also known as BHLHB2, STRA13, DEC1, or SHARP2, is a member of the basic helix-loop-helix (bHLH) protein family, a large superfamily of transcriptional regulators expressed in many organisms. BHLHE40 is known to regulate a wide variety of essential cellular processes, including cell cycle, cellular proliferation, programmed cell death, cellular development and differentiation, as well as circadian rhythms (PMID: 34551158). It is reported that BHLHE40 is overexpressed in gastric, breast, and brain tumors; and downregulated in colorectal, esophageal, pancreatic and lung cancer (PMID: 32577154).

实验方案

Product Specific Protocols
IHC protocol for BHLHE40 antibody 17895-1-APDownload protocol
IP protocol for BHLHE40 antibody 17895-1-APDownload protocol
WB protocol for BHLHE40 antibody 17895-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Immunity

Progranulin deficiency-induced lysosomal dysfunction drives maladaptive myeloid cell states through the MITF/TFE transcription factors

Authors - Leon Tejwani
View Article

Cancer Discov

Senescent-like neutrophils shape angiogenic immunosuppressive niches in colorectal cancer liver metastasis.

Authors - Zhihang Chen
View Article
humanWB

Cancer Res

Single-Cell and Spatial Transcriptome Profiling Identifies the Transcription Factor BHLHE40 as a Driver of EMT in Metastatic Colorectal Cancer

Authors - Sheng Yang
  • KD Validated
View Article
humanWB

Adv Sci (Weinh)

The PRMT6/PARP1/CRL4B Complex Regulates the Circadian Clock and Promotes Breast Tumorigenesis

Authors - Tianshu Yang
View Article
humanIF

Adv Sci (Weinh)

BHLHE40 Cooperates with GATA2/3 to Control Human Syncytiotrophoblast Lineage Differentiation.

Authors - Lijin Peng
View Article

Cell Commun Signal

BMAL1/p53 mediating bronchial epithelial cell autophagy contributes to PM2.5-aggravated asthma

Authors - Shuai-Jun Chen
View Article
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