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MIST1 Polyclonal antibody

MIST1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 25140-1-AP

Print datasheet

Synonyms

BHLHA15, BHLHB8, MIST 1, MIST1

验证数据展示

  • WB analysis of mouse liver using 25140-1-AP

    WB analysis of mouse liver using 25140-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25140-1-AP (MIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 25140-1-AP

    IP experiment of mouse liver using 25140-1-AP

    IP result of anti-MIST1 (IP:25140-1-AP, 3ug; Detection:25140-1-AP 1:300) with mouse liver tissue lysate 6800ug.

经过测试的应用

Positive WB detected inmouse liver tissue, rat pancreas tissue
Positive IP detected inmouse liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25140-1-AP is MIST1 Fusion Protein expressed in E. coli.

Tested Applications IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen MIST1 fusion protein Ag18199 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name basic helix-loop-helix family, member a15
Synonyms BHLHA15, BHLHB8, MIST 1, MIST1
Calculated Molecular Weight 189 aa, 21 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC113394
Gene Symbol BHLHA15
Gene ID (NCBI) 168620
RRIDAB_2879920
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7RTS1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MIST1 antibody 25140-1-APDownload protocol
IP protocol for MIST1 antibody 25140-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols