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验证数据展示

  • WB analysis of mouse liver using 25140-1-AP

    WB analysis of mouse liver using 25140-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25140-1-AP (MIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 25140-1-AP

    IP experiment of mouse liver using 25140-1-AP

    IP result of anti-MIST1 (IP:25140-1-AP, 3ug; Detection:25140-1-AP 1:300) with mouse liver tissue lysate 6800ug.

MIST1 Polyclonal antibody

MIST1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25140-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

BHLHA15, BHLHB8, MIST 1, MIST1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, rat pancreas tissue
Positive IP detected inmouse liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25140-1-AP targets MIST1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MIST1 fusion protein Ag18199 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 basic helix-loop-helix family, member a15
别名 BHLHA15, BHLHB8, MIST 1, MIST1
计算分子量 189 aa, 21 kDa
观测分子量 30 kDa
GenBank蛋白编号BC113394
基因名称 MIST1
Gene ID (NCBI) 168620
RRIDAB_2879920
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7RTS1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MIST1 antibody 25140-1-APDownload protocol
IP protocol for MIST1 antibody 25140-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols