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验证数据展示

  • WB analysis using 17492-1-AP

    WB analysis using 17492-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 17492-1-AP (Filensin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17492-1-AP

    IF Staining of HeLa using 17492-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Filensin antibody (17492-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Filensin Polyclonal antibody

Filensin Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17492-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

BFSP1, LIFL-H, Lens intermediate filament-like heavy, Lens fiber cell beaded-filament structural protein CP 115, Filensin N-terminal fragment

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse eye tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17492-1-AP targets Filensin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag11591

Product name: Recombinant human BFSP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 192-540 aa of BC041483

Sequence: GNRLTSAFIETPIPLFTQSHGVSLSTGSGGKDLTRALQDITAAKPRQKALPKNVPRRKEIITKDKTNGALEDAPLKGLEDTKLVQVVLKEESESKFESESKEVSPLTQEGAPEDVPDGGQISKGFGKLYRKVKEKVRSPKEPETPTELYTKERHVLVTGDANYVDPRFYVSSITAKGGVAVSVAEDSVLYDGQVEPSPESPKPPLENGQVGLQEKEDGQPIDQQPIDKEIEPDGAELEGPEEKREGEERDEESRRPCAMVTPGAEEPSIPEPPKPAADQDGAEVLGTRSRSLPEKGPPKALAYKTVEVVESIEKISTESIQTYEETAVIVETMIGKTKSDKKKSGEKSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 beaded filament structural protein 1, filensin
别名 BFSP1, LIFL-H, Lens intermediate filament-like heavy, Lens fiber cell beaded-filament structural protein CP 115, Filensin N-terminal fragment
计算分子量540aa,60 kDa; 665aa,75 kDa
观测分子量 94 kDa
GenBank蛋白编号BC041483
基因名称 Filensin
Gene ID (NCBI) 631
RRIDAB_2228016
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12934
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for Filensin antibody 17492-1-APDownload protocol
WB protocol for Filensin antibody 17492-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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