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验证数据展示

  • WB analysis of mouse testis using 24711-1-AP

    WB analysis of mouse testis using 24711-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24711-1-AP (BEND4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 24711-1-AP

    IHC staining of human heart using 24711-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24711-1-AP (BEND4 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human colon cancer using 24711-1-AP

    IHC staining of human colon cancer using 24711-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24711-1-AP (BEND4 Antibody) at dilution of 1:50 (under 40x lens).

BEND4 Polyclonal antibody

BEND4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24711-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

BEN domain containing 4, BEND4, CCDC4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman colon cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24711-1-AP targets BEND4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 BEND4 fusion protein Ag20169 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BEN domain containing 4
别名 BEN domain containing 4, BEND4, CCDC4
计算分子量 534 aa, 58 kDa
观测分子量 55-58 kDa, 33-36 kDa
GenBank蛋白编号BC128592
基因名称 BEND4
Gene ID (NCBI) 389206
RRIDAB_2879684
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZU67
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BEND4, also named as CCDC4, has 5 isoforms with MW 55-58 kDa, 45-48 kDa and 36 kDa.

实验方案

Product Specific Protocols
WB protocol for BEND4 antibody 24711-1-APDownload protocol
IHC protocol for BEND4 antibody 24711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols