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验证数据展示

  • WB analysis using 87001-4-RR

    WB analysis using 87001-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87001-4-RR (BEND3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity of 87001-4-RR

    Affinity of 87001-4-RR

    Biolayer interferometry (BLl) kinetic assays of 87001-4-RR against Human BEND3 were performed. The affinity constant is 6.74 nM.

BEND3 Recombinant monoclonal antibody

BEND3 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 87001-4-RR

产品说明书

CloneNo. 252025C12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

BEN domain containing 3, BEN domain-containing protein 3, KIAA1553, RP11 59I9.2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, L02 cells, HEK-293 cells, HepG2 cells, U2OS cells, mouse embryo

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87001-4-RR targets BEND3 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag19137

Product name: Recombinant human BEND3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 493-828 aa of BC131628

Sequence: PPRDDCYDSSSLPDDISVVKVEDSFEGERPGRRSKKIWLVPIDFDKLEIPQPDFEVPGADCLLSKEQLRSIYESSLSIGNFASRLLVHLFPELFTHENLRKQYSCSGSLGKKQLDPSRIKLIRHYVQLLYPRAKNDRVWTLEFVGKLDERCRRRDTEQRRSYQQQRKVHVPGPECRDLTSYAINPERFREEFEGPPLPPERSSKDFCKIPLDELVVPSPDFPVPSPYLLSDKEVREIVQQSLSVGNFAARLLVRLFPELFTAENLRLQYNHSGACNKKQLDPTRLRLIRHYVEAVYPVEKMEEVWHYECIPSIDERCRRPNRKKCDILKKAKKVEK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BEN domain containing 3
别名 BEN domain containing 3, BEN domain-containing protein 3, KIAA1553, RP11 59I9.2
计算分子量 828 aa, 94 kDa
观测分子量94-110 kDa
GenBank蛋白编号BC131628
基因名称 BEND3
Gene ID (NCBI) 57673
RRIDAB_3745266
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ5T5X7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BEND3 is a quadruple BEN domain-containing protein that associates with heterochromatin and functions as a transcriptional repressor. BEND3 is highly expressed in pluripotent cells, and the induction of differentiation results in the down-regulation of BEND3. (PMID: 35217604, PMID: 25600804)

实验方案

Product Specific Protocols
WB protocol for BEND3 antibody 87001-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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