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验证数据展示

  • WB analysis using 23101-1-AP

    WB analysis using 23101-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23101-1-AP (BEND3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 23101-1-AP

    IF Staining of HEK-293 using 23101-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using BEND3 antibody (23101-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

BEND3 Polyclonal antibody

BEND3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 23101-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

BEN domain containing 3, BEN domain-containing protein 3, KIAA1553, RP11 59I9.2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, NCCIT cells, U2OS cells
Positive IF/ICC detected inHEK-293 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23101-1-AP targets BEND3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19137

Product name: Recombinant human BEND3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 493-828 aa of BC131628

Sequence: PPRDDCYDSSSLPDDISVVKVEDSFEGERPGRRSKKIWLVPIDFDKLEIPQPDFEVPGADCLLSKEQLRSIYESSLSIGNFASRLLVHLFPELFTHENLRKQYSCSGSLGKKQLDPSRIKLIRHYVQLLYPRAKNDRVWTLEFVGKLDERCRRRDTEQRRSYQQQRKVHVPGPECRDLTSYAINPERFREEFEGPPLPPERSSKDFCKIPLDELVVPSPDFPVPSPYLLSDKEVREIVQQSLSVGNFAARLLVRLFPELFTAENLRLQYNHSGACNKKQLDPTRLRLIRHYVEAVYPVEKMEEVWHYECIPSIDERCRRPNRKKCDILKKAKKVEK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BEN domain containing 3
别名 BEN domain containing 3, BEN domain-containing protein 3, KIAA1553, RP11 59I9.2
计算分子量 828 aa, 94 kDa
观测分子量94 kDa
GenBank蛋白编号BC131628
基因名称 BEND3
Gene ID (NCBI) 57673
RRIDAB_2879210
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ5T5X7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BEND3 is a quadruple BEN domain-containing protein that associates with heterochromatin and functions as a transcriptional repressor. BEND3 is highly expressed in pluripotent cells, and the induction of differentiation results in the down-regulation of BEND3. (PMID: 35217604, PMID: 25600804)

实验方案

Product Specific Protocols
IF protocol for BEND3 antibody 23101-1-APDownload protocol
WB protocol for BEND3 antibody 23101-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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