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验证数据展示

  • WB analysis using 23101-1-AP

    WB analysis using 23101-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23101-1-AP (BEND3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 23101-1-AP

    WB analysis of HEK-293 using 23101-1-AP

    WB result of BEND3 antibody (23101-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-BEND3 transfected HEK-293 cells.

  • IF Staining of HEK-293 using 23101-1-AP

    IF Staining of HEK-293 using 23101-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using BEND3 antibody (23101-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • KD/KO Validated

BEND3 Polyclonal antibody

BEND3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 23101-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

BEN domain containing 3, BEN domain-containing protein 3, KIAA1553, RP11 59I9.2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, NCCIT cells, U2OS cells
Positive IF/ICC detected inHEK-293 cells, HeLa cells, A431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

23101-1-AP targets BEND3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag19137

Product name: Recombinant human BEND3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 493-828 aa of BC131628

Sequence: PPRDDCYDSSSLPDDISVVKVEDSFEGERPGRRSKKIWLVPIDFDKLEIPQPDFEVPGADCLLSKEQLRSIYESSLSIGNFASRLLVHLFPELFTHENLRKQYSCSGSLGKKQLDPSRIKLIRHYVQLLYPRAKNDRVWTLEFVGKLDERCRRRDTEQRRSYQQQRKVHVPGPECRDLTSYAINPERFREEFEGPPLPPERSSKDFCKIPLDELVVPSPDFPVPSPYLLSDKEVREIVQQSLSVGNFAARLLVRLFPELFTAENLRLQYNHSGACNKKQLDPTRLRLIRHYVEAVYPVEKMEEVWHYECIPSIDERCRRPNRKKCDILKKAKKVEK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BEN domain containing 3
别名 BEN domain containing 3, BEN domain-containing protein 3, KIAA1553, RP11 59I9.2
计算分子量 828 aa, 94 kDa
观测分子量94 kDa
GenBank蛋白编号BC131628
基因名称 BEND3
Gene ID (NCBI) 57673
RRIDAB_2879210
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ5T5X7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BEND3 is a quadruple BEN domain-containing protein that associates with heterochromatin and functions as a transcriptional repressor. BEND3 is highly expressed in pluripotent cells, and the induction of differentiation results in the down-regulation of BEND3. (PMID: 35217604, PMID: 25600804)

实验方案

Product Specific Protocols
IF protocol for BEND3 antibody 23101-1-APDownload protocol
WB protocol for BEND3 antibody 23101-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Res

TRAPPC4 Promotes GPX4 Stability to Drive Ferroptosis Resistance and Tumor Progression in Head and Neck Squamous Cell Carcinoma

Authors - Zhao Ding
View Article
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