验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24958-1-AP targets BEND2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag21152 Product name: Recombinant human BEND2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 298-645 aa of BC096745 Sequence: FHPNLESGPQMSYGTMSYSTEMKNNCDQDDASASACLTPDFALLPLNILVEVDTNTENSVNTMNRSTLLDSDSGQDSSSSSVCIPPKYGYLGDPKRNVRVLKIHLLAVQNMAKPKQAACYLVRILFSKEILISSSVDIHLKDSQSLDPNKMAALREYLATTFPTCDLHEHGKDWQDCISGINSMIYCLCSEGKSTPKTVRKNKKRTNRVASASADRNDQRGRDGGEGCSWMFQPMNNSKMREKRNLQPNSNAIPEGMREPSTDNPEEPGEAWSYFGRPWRNIRMPCSVLTLAKTKSCASLSARYLIQKLFTKDVLVQSNVYGNLKHGLCALDPNKISALRGLLHIEYV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | BEN domain containing 2 |
| 别名 | BEN domain containing 2, CXorf20 |
| 计算分子量 | 799 aa, 88 kDa |
| 观测分子量 | 130-140 kDa |
| GenBank蛋白编号 | BC096745 |
| 基因名称 | BEND2 |
| Gene ID (NCBI) | 139105 |
| RRID | AB_3742131 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NDZ0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BEND2 (BEN domain-containing protein 2) is a novel protein that is specifically expressed in spermatogenic cells around the time of meiotic initiation (PMID: 35613276). BEND2 interacted with a number of chromatin-associated proteins-including ZMYM2, LSD1, CHD4, and ADNP- which are components of certain transcription-repressor complexes. In the testis, Bend2 expresses two protein isoforms: the 130 kDa protein corresponds to the full-length BEND2, which displays a slower electrophoretic mobility due to its unusual sequence/structure. The 75 kDa protein corresponds to a smaller version of BEND2 lacking the N-terminus, p80 .
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BEND2 antibody 24958-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
