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验证数据展示

  • WB analysis of U-251 using 66292-1-Ig (same clone as 66292-1-PBS)

    WB analysis of U-251 using 66292-1-Ig (same clone as 66292-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 66292-1-Ig (BDNF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66292-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 66292-1-Ig (same clone as 66292-1-PBS)

    IF Staining of U-251 using 66292-1-Ig (same clone as 66292-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using BDNF antibody (66292-1-Ig, Clone: 1E5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66292-1-PBS in a different storage buffer formulation.

BDNF Monoclonal antibody, PBS Only

BDNF Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66292-1-PBS

产品说明书

CloneNo. 1E5C5

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

Brain-derived neurotrophic factor, brain derived neurotrophic factor, BDNF precursor form, 1E5C5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66292-1-PBS targets BDNF in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag11329

Product name: Recombinant human BDNF protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 21-247 aa of BC029795

Sequence: MKEANIRGQGGLAYPGVRTHGTLESVNGPKAGSRGLTSLADTFEHMIEELLDEDQKVRPNEENNKDADLYTSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 brain-derived neurotrophic factor
别名 Brain-derived neurotrophic factor, brain derived neurotrophic factor, BDNF precursor form, 1E5C5
计算分子量 247 aa, 28 kDa
观测分子量 32 kDa
GenBank蛋白编号BC029795
基因名称 BDNF
Gene ID (NCBI) 627
RRIDAB_2881675
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP23560
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).

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