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验证数据展示

  • WB analysis of pig liver using 67448-1-Ig (same clone as 67448-1-PBS)

    WB analysis of pig liver using 67448-1-Ig (same clone as 67448-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 67448-1-Ig (same clone as 67448-1-PBS)

    WB analysis of HSC-T6 using 67448-1-Ig (same clone as 67448-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 67448-1-Ig (same clone as 67448-1-PBS)

    WB analysis of MCF-7 using 67448-1-Ig (same clone as 67448-1-PBS)

    WB result of BDH1 antibody (67448-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BDH1 transfected MCF-7 cells. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67448-1-Ig (same clone as 67448-1-PBS)

    IHC staining of human liver cancer using 67448-1-Ig (same clone as 67448-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67448-1-Ig (BDH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BDH1 antibody included
  • IHC staining of human liver cancer using 67448-1-Ig (same clone as 67448-1-PBS)

    IHC staining of human liver cancer using 67448-1-Ig (same clone as 67448-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67448-1-Ig (BDH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BDH1 antibody included
  • IF Staining of mouse liver using 67448-1-Ig (same clone as 67448-1-PBS)

    IF Staining of mouse liver using 67448-1-Ig (same clone as 67448-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BDH1 antibody (67448-1-Ig, Clone: 2C3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 67448-1-Ig (same clone as 67448-1-PBS)

    IF Staining of mouse liver using 67448-1-Ig (same clone as 67448-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BDH1 antibody (67448-1-Ig, Clone: 2C3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

BDH1 Monoclonal antibody, PBS Only

BDH1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67448-1-PBS

产品说明书

CloneNo. 2C3D4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, ELISA

别名

2C3D4, 3-hydroxybutyrate dehydrogenase, BDH, D-beta-hydroxybutyrate dehydrogenase, mitochondrial, EC:1.1.1.30

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BDH1 Ready-To-Use IHC Kit. BDH1 primary antibody included.

产品信息

67448-1-PBS targets BDH1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag7864

Product name: Recombinant human BDH1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-343 aa of BC005844

Sequence: MLATRLSRPLSRLPGKTLSACDRENGARRPLLLGSTSFIPIGRRTYASAAEPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLNSDRLRTVQLNVCSSEEVEKVVEIVRSSLKDPEKGMWGLVNNAGISTFGEVEFTSLETYKQVAEVNLWGTVRMTKSFLPLIRRAKGRVVNISSMLGRMANPARSPYCITKFGVEAFSDCLRYEMYPLGVKVSVVEPGNFIAATSLYSPESIQAIAKKMWEELPEVVRKDYGKKYFDEKIAKMETYCSSGSTDTSPVIDAVTHALTATTPYTRYHPMDYYWWLRMQIMTHLPGAISDMIYIR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 3-hydroxybutyrate dehydrogenase, type 1
别名 2C3D4, 3-hydroxybutyrate dehydrogenase, BDH, D-beta-hydroxybutyrate dehydrogenase, mitochondrial, EC:1.1.1.30
计算分子量 38 kDa
观测分子量 31 kDa
GenBank蛋白编号BC005844
基因名称 BDH1
Gene ID (NCBI) 622
RRIDAB_2882682
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ02338
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).

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