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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Jurkat using 67260-1-Ig (same clone as 67260-1-PBS)

    WB analysis of Jurkat using 67260-1-Ig (same clone as 67260-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67260-1-Ig (BCR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67260-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 67260-1-Ig (same clone as 67260-1-PBS)

    FC experiment of K-562 using 67260-1-Ig (same clone as 67260-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human BCR (67260-1-Ig, Clone:2C2E3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67260-1-PBS in a different storage buffer formulation.

BCR Monoclonal antibody, PBS Only

BCR Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67260-1-PBS

产品说明书

CloneNo. 2C2E3

宿主/亚型

Mouse / IgG2b

种属反应性

Human, rat

应用

WB, Indirect ELISA

别名

ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, FLJ16453, PHL

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BCR Ready-To-Use IHC Kit. BCR primary antibody included.

产品信息

67260-1-PBS targets BCR in WB, Indirect ELISA applications and shows reactivity with Human, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human, rat
免疫原

CatNo: Ag28953

Product name: Recombinant human BCR protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 286-494 aa of BC066122

Sequence: GMMEGEGKGPLLRSQSTSEQEKRLTWPRRSYSPRSFEDCGGGYTPDCSSNENLTSSEEDFSSGQSSRVSPSPTTYRMFRDKSRSPSQNSQQSFDSSSPPTPQCHKRHRHCPVVVSEATIVGVRKTGQIWPNDGEGAFHGDADGSFGTPPGYGCAADRAEEQRRHQDGLPYIDDSPSSSPHLSSKGRGSRDALVSGALESTKASELDLEK

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 breakpoint cluster region
别名 ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, FLJ16453, PHL
计算分子量 143 kDa
观测分子量 150-160 kDa
GenBank蛋白编号BC066122
基因名称 BCR
Gene ID (NCBI) 613
RRIDAB_2882531
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP11274
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.