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验证数据展示

  • WB analysis using 24315-1-AP (same clone as 24315-1-PBS)

    WB analysis using 24315-1-AP (same clone as 24315-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24315-1-AP (BCORL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24315-1-PBS in a different storage buffer formulation.

  • WB analysis using 24315-1-AP (same clone as 24315-1-PBS)

    WB analysis using 24315-1-AP (same clone as 24315-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24315-1-AP (BCORL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24315-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 24315-1-AP (same clone as 24315-1-PBS)

    IF Staining of U2OS using 24315-1-AP (same clone as 24315-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using BCORL1 antibody (24315-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 24315-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 24315-1-AP (same clone as 24315-1-PBS)

    IHC staining of mouse testis using 24315-1-AP (same clone as 24315-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24315-1-AP (BCORL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24315-1-PBS in a different storage buffer formulation.

BCORL1 Polyclonal antibody, PBS Only

BCORL1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 24315-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24315-1-PBS targets BCORL1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag19522

Product name: Recombinant human BCORL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-349 aa of BC156742

Sequence: MISTAPLYSGVHNWTSSDRIRMCGINEERRAPLSDEESTTGDCQHFGSQEFCVSSSFSKVELTAVGSGSNARGADPDGSATEKLGHKSEDKPDDPQPKMDYAGNVAEAEGFLVPLSSPGDGLKLPASDSAEASNSRADCSWTPLNTQMSKQVDCSPAGVKALDSRQGVGEKNTFILATLGTGVPVEGTLPLVTTNFSPLPAPICPPAPGSASVPHSVPDAFQVPLSVPAPVPHSGLVPVQVATSVPAPSPPLAPVPALAPAPPSVPTLISDSNPLSVSASVLVPVPASAPPSGPVPLSAPAPAPLSVPVSAPPLALIQAPVPPSAPTLVLAPVPTPVLAPMPASTPPAA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BCL6 co-repressor-like 1
别名
计算分子量 1711 aa, 183 kDa
观测分子量200 kDa
GenBank蛋白编号BC156742
基因名称 BCORL1
Gene ID (NCBI) 63035
RRIDAB_3669432
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5H9F3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BCORL1 is a transcriptional corepressor found tethered to promoter regions by DNA-binding proteins. BCORL1 can interact with class II histone deacetylases, and interact with the CtBP corepressor through the CtBP binding motif-PXDLS (PMID: 21989985, 17379597). The open reading frame of BCORL1 cDNA encodes a protein of 1711 amino acids. The antibody recognized a band migrating through SDS-polyacrylamide gels at a size of ∼200 kDa.

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