验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, K-562 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12107-1-AP targets BCOR in WB, IHC, IP, CoIP, ChIP, ELISA, CUT&RUN applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, CoIP, ChIP, CUT&RUN |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2752 Product name: Recombinant human BCOR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-361 aa of BC009675 Sequence: RFRKRPEPSSDYDLSPAKQEPKPFDRLQQLLPASQSTQLPCSSSPQETTQSRPMPPEARRLIVNKNAGETLLQRAARLGYEEVVLYCLENKICDVNHRDNAGYCALHEACARGWLNIVRHLLEYGADVNCSAQDGTRPLHDAVENDHLEIVRLLLSYGADPTLATYSGRTIMKMTHSELMEKFLTDYLNDLQGRNDDDASGTWDFYGSSVCEPDDESGYDVLANPPGPEDQDDDDDAYSDVFEFEFSETPLLPCYNIQVSVAQGPRNWLLLSDVLKKLKMSSRIFRCNFPNVEIVTIAEAEFYRQVSASLLFSCSKDLEAFNPESKELLDLVEFTNEIQTLLGSSVEWLHPSDLASDNYW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | BCL6 co-repressor |
| 别名 | ANOP2, BCOR, KIAA1575, MAA2, MCOPS2 |
| 计算分子量 | 1703 aa, 186 kDa |
| 观测分子量 | 186 kDa |
| GenBank蛋白编号 | BC009675 |
| 基因名称 | BCOR |
| Gene ID (NCBI) | 54880 |
| RRID | AB_2290335 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6W2J9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BCOR, a corepressor of BCL6, binds to transcriptional factors and specifically inhibits gene expression. BCOR plays a key role in the early embryonic development, mesenchymal stem cell function and hematopiesis. BCOR can associate with HDACs, the polycomb group protein, polycomb group Ring finger protein1/nervous system polycomb1 (PCGF1/NSPC1), histone demethylase, F-box- and leucine-rich repeat protein10(FBXL10). Via repression of TFAP2A acts as a negative regulator of osteo-dentiogenic capacity in adult stem
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for BCOR antibody 12107-1-AP | Download protocol |
| IP protocol for BCOR antibody 12107-1-AP | Download protocol |
| WB protocol for BCOR antibody 12107-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Genet Consistent in-frame internal tandem duplications of BCOR characterize clear cell sarcoma of the kidney. | ||
Blood Epigenetic Regulation of Non-canonical Menin Targets Modulates Menin Inhibitor Response in Acute Myeloid Leukemia | ||
Nat Commun PCGF1-PRC1 links chromatin repression with DNA replication during hematopoietic cell lineage commitment | ||
Mol Cell Non-canonical PRC1.1 licenses transcriptional response to enable Treg plasticity in immune adaptation | ||
Mol Cell SKP1A bound to Polycomb-silenced genes mediates degradation of PRC2 and preconditions their activation. | ||
Nucleic Acids Res Sustained PRC1.1 activity preserves gene repression independently of PRC2.2 and restrains leukemic cell differentiation. |
