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  • KD/KO Validated

BCLAF1 Polyclonal antibody

BCLAF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 26809-1-AP

Print datasheet

Synonyms

BCLAF1, bK211L9.1, BTF, KIAA0164



经过测试的应用

Positive WB detected inJurkat cells, MCF-7 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy BCLAF1 Ready-To-Use IHC Kit. BCLAF1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26809-1-AP targets BCLAF1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Immunogen BCLAF1 fusion protein Ag25210 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BCL2-associated transcription factor 1
Synonyms BCLAF1, bK211L9.1, BTF, KIAA0164
Calculated Molecular Weight 920 aa, 106 kDa
Observed Molecular Weight 145 kDa
GenBank Accession NumberBC132780
Gene Symbol BCLAF1
Gene ID (NCBI) 9774
RRIDAB_2880642
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NYF8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCLF1, also named as Bcl-2-associated transcription factor 1, is a 920 amino acid protein, which Interacts with Bcl-2 related proteins, EMD, with the adenovirus E1B 19 kDa protein and with DNA. BCLF1 as a death-promoting transcriptional repressor may be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. The calculated molecular weight of BCLF1 is a 110 kDa, but the modified BCLF1 protein is about 140-150 kDa.

实验方案

Product Specific Protocols
WB protocol for BCLAF1 antibody 26809-1-APDownload protocol
IHC protocol for BCLAF1 antibody 26809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Nucleic Acids Res

The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.

Authors - Xin Zong
humanWB

Aging (Albany NY)

LncRNA PVT1 accelerates malignant phenotypes of bladder cancer cells by modulating miR-194-5p/BCLAF1 axis as a ceRNA.

Authors - Mingwei Chen
humanWB,IHC,IF

Transl Oncol

Hsp90 Inhibitor STA9090 induced VPS35 related extracellular vesicle release and metastasis in hepatocellular carcinoma

Authors - Wenchong Tan
  • KD Validated
mouseWB

eLife

SRSF10 is essential for progenitor spermatogonia expansion by regulating alternative splicing

Authors - Wenbo Liu
humanWB

J Oncol

The Comprehensive Analysis of N6-Methyadenosine Writer METTL3 and METTL14 in Gastric Cancer

Authors - Yiling Ge
humanWB

Biochem Genet

BCLAF1 is Expressed as a Potential Anti-oncogene in Bile Duct Cancer

Authors - Yutong Zhou