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验证数据展示

  • IF Staining of HeLa using 83760-3-RR

    IF Staining of HeLa using 83760-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BCL9 antibody (83760-3-RR, Clone: 240682A9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of SH-SY5Y using 83760-3-RR

    FC experiment of SH-SY5Y using 83760-3-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Bcl9 Recombinant Antibody (83760-3-RR, Clone:240682A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83760-3-RR

    Affinity and Kinetic Characterization of 83760-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83760-3-RR against Human BCL9 were performed. The affinity constant is 5.39 nM.

BCL9 Recombinant monoclonal antibody

BCL9 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83760-3-RR

产品说明书

CloneNo. 240682A9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

Bcl-9, Bcl 9, B-cell lymphoma 9 protein, B-cell CLL/lymphoma 9 protein, B cell CLL/lymphoma 9

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83760-3-RR targets BCL9 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19158

Product name: Recombinant human BCL9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 8-340 aa of BC116451

Sequence: VRSSPSGNTQSSPKSKQEVMVRPPTVMSPSGNPQLDSKFSNQGKQGGSASQSQPSPCDSKSGGHTPKALPGPGGSMGLKNGAGNGAKGKGKRERSISADSFDQRDPGTPNDDSDIKECNSADHIKSQDSQHTPHSMTPSNATAPRSSTPSHGQTTATEPTPAQKTPAKVVYVFSTEMANKAAEAVLKGQVETIVSFHIQNISNNKTERSTAPLNTQISALRNDPKPLPQQPPAPANQDQNSSQNTRLQPTPPIPAPAPKPAAPPRPLDRESPGVENKLIPSVGSPASSTPLPPDGTGPNSTPNNRAVTPVSQGSNSSSADPKAPPPPPVSSGE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B-cell CLL/lymphoma 9
别名 Bcl-9, Bcl 9, B-cell lymphoma 9 protein, B-cell CLL/lymphoma 9 protein, B cell CLL/lymphoma 9
计算分子量 1426 aa, 149 kDa
GenBank蛋白编号BC116451
基因名称 BCL9
Gene ID (NCBI) 607
RRIDAB_3671356
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO00512
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.

实验方案

Product Specific Protocols
IF protocol for BCL9 antibody 83760-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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